Tools, Techniques and Assessment in Biology

Tools, Techniques and Assessment in Biology PDF

Author: John Adds

Publisher: Nelson Thornes

Published: 1999

Total Pages: 164

ISBN-13: 9780174482734

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Nelson Advanced Science Biology is a complete series of lively, high quality, affordable student books for senior secondary students of Biology and Human Biology.

Molecular Biology Techniques

Molecular Biology Techniques PDF

Author: Heather B. Miller

Publisher: Academic Press

Published: 2011-10-18

Total Pages: 227

ISBN-13: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Techniques and Methods in Biology

Techniques and Methods in Biology PDF

Author: Ghatak K. L.

Publisher: PHI Learning Pvt. Ltd.

Published: 2010-12-30

Total Pages: 691

ISBN-13: 8120341716

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This comprehensive and accessible text is aimed at undergraduate and postgraduate students of biotechnology, and postgraduate students of botany and zoology. The book discusses spectroscopy, which forms the core of modern research, and microscopy, which is now an indispensable analytical tool. It also illustrates radioactivity and related phenomena, and evaluates the role of statistics.

Tools and Techniques in Biomolecular Science

Tools and Techniques in Biomolecular Science PDF

Author: Aysha Divan

Publisher: Oxford University Press

Published: 2013-03-21

Total Pages: 529

ISBN-13: 0199695563

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This book reviews the theoretical concepts and experimental details underpinning the broad range of modern technologies that are currently being used to advance our understanding of the biomolecular sciences.

Biophysical Tools for Biologists

Biophysical Tools for Biologists PDF

Author: John J. Correia

Publisher: Academic Press

Published: 2011-09-21

Total Pages: 996

ISBN-13: 0080554946

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Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. Describes techniques in the context of important biological problems Delineates critical steps and potential pitfalls for each method Includes full-color plates to illustrate techniques

Analytical Techniques in Biosciences

Analytical Techniques in Biosciences PDF

Author: Chukwuebuka Egbuna

Publisher: Academic Press

Published: 2021-10-21

Total Pages: 302

ISBN-13: 0128227990

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Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry. The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. Presents basic analytical protocols and sample-preparation guidelines Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research Presents biostatistical tools and methods and basic computational models in biosciences

Biophysical Methods Tools and Techniques in Biology

Biophysical Methods Tools and Techniques in Biology PDF

Author: Dr. Nisha Raghav, Dr. Ravindra Pratap Raghava

Publisher: Notion Press

Published: 2017-11-17

Total Pages: 128

ISBN-13: 1948147505

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The book covers three areas: Historical background, optical principles involved in diffraction and image formation in the light microscope; the basic modes of light microscopy and electron microscopy; the components of modern electronic imaging systems and the basic image-processing operations that are required to prepare an image. Each chapter is introduced with theory regarding the topic at hand, followed by descriptions of instrument alignment and image interpretation. As a biologist and a practitioner of microscopy, the reader will observe that the book is focused on how to align and operate microscopes with an abbreviated treatment to the physical theory and principles involved. The content is not difficult, so the demonstrations are valuable aids in absorbing essential optical principles. They also allow time to pause and reflect on the economy and aesthetic beauty of optical laws and principles. If carried out, the demonstrations and exercises also offer opportunities to help the reader become acquainted with new biological specimens using a new mode of light microscopy. . A basic glossary has also been included to aid readers not already familiar with complex terminology.

Advances in Protein Molecular and Structural Biology Methods

Advances in Protein Molecular and Structural Biology Methods PDF

Author: Timir Tripathi

Publisher: Academic Press

Published: 2022-01-14

Total Pages: 716

ISBN-13: 0323902650

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Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. Presents a thorough overview of the latest and emerging methods and technologies for protein study Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy Includes computational and machine learning methods Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins