The Glass Knife

The Glass Knife PDF

Author: John Tully

Publisher: John Tully

Published:

Total Pages: 97

ISBN-13:

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A powerful story set in the strange world of pre-Columbian South America. The Tio-Cada is the ‘Chosen One’ — chosen to take part in the Miracle of Re-Birth. On the Day of the Spider he will be sacrificed on the altar of the Sun-God, his heart cut from his body and displayed to the multitude . . . only to appear again next morning restored to life. Tio, as he is known to his friends, has lived his life preparing to take part in the ceremony but when one of those friends, more knowledgeable than the others, warns him, ‘gods are fickle and priests are cunning’ he begins to wonder if the Miracle of Re-Birth is as straightforward as it seems? His desire to satisfy his curiosity leads him into strange adventures and remarkable discoveries.

Biological Electron Microscopy

Biological Electron Microscopy PDF

Author: Michael J. Dykstra

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 368

ISBN-13: 146840010X

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In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.

A Manual of Applied Techniques for Biological Electron Microscopy

A Manual of Applied Techniques for Biological Electron Microscopy PDF

Author: Michael J. Dykstra

Publisher: Springer Science & Business Media

Published: 1993-08-31

Total Pages: 280

ISBN-13: 9780306444494

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This easy-to-follow manual describes tested procedures used to prepare biological samples for scanning and transmission electron microscopy, as well as methods for cytochemistry, immunocytochemistry, and scientific photography. The work is structured to clearly define testing objectives, necessary materials, procedural steps, and expected results; a list of references and trouble shooting techniques round out the text.

Fine Structure Immunocytochemistry

Fine Structure Immunocytochemistry PDF

Author: Gareth Griffiths

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 477

ISBN-13: 3642770959

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Electron microscopy in the biological sciences can be divided into two disciplines. The first, concerned with high resolution detail of particles or periodic structures, is mostly based on sound theoretical principles of physics. The second, by far the larger discipline, is interested in the information obtainable from thin sections. The theoretical back ground to those groups of techniques for preparing and looking at thin sections is often inexact and "loose", for want of a better word. What should be chemistry is often closer to alchemy. This kind of electron microscopy is often enshrined with mystical recipes, handed down from generation to generation. Admittedly, many of the processes involved, such as those required to embed tissue in epoxy resins, involve multiple interconnected steps, which make it difficult to follow the details of anyone of these steps. If all these steps are shrouded in some mystery, however, can one really trust the final image that emerges on the EM screen? When we present the data in some semi quantitative form is there really no better way to do it than to categorize the parameters with ++, +/-, etc? What happens when one labels the sections with antibodies? Does the whole business necess arily need to be more of an "art" than a "science"? Upon reflecting on these problems in 1981, I had the impression that many of the multi-authored textbooks that existed then (and that have appeared since) tended to exacerbate or at least perpetuate this

Introduction to Electron Microscopy

Introduction to Electron Microscopy PDF

Author: Saul Wischnitzer

Publisher: Elsevier

Published: 2013-10-22

Total Pages: 311

ISBN-13: 1483148696

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Introduction to Electron Microscopy, Second Edition provides an introduction to the foundations of electron microscopy; an outline of some practical aspects of instrument operation; and discussion of the rationale of the methodology of biological specimen preparation. The book seeks to provide a comprehensive understanding of the theoretical and operational aspects of the electron microscope. This edition consists of two parts. Part One deals with the history, basic theory, and operation of the electron microscope. Part Two discusses steps used in material preparation for electron microscope investigation such as fixation, embedding, and staining techniques. Biomedical researchers, molecular biologists, toxicologists, forensic investigators, and medical students will find this book a very useful reference.

Polymer Mixing and Extrusion Technology

Polymer Mixing and Extrusion Technology PDF

Author: NicholasP. Cheremisinoff

Publisher: Routledge

Published: 2017-10-02

Total Pages: 456

ISBN-13: 1351423509

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Addressing the two major unit operations-mixing and extrusion-fundamental toprocessing elastomers and plastic materials, this reference summarizes design equationsthat can be employed effectively in scaling up product performance parameters, andcontains a thorough survey of rheological principles. In addition, the book provides awealth of practical information, relating molecular and compositional properties ofpolymers to processing characteristics and end-use properties so that engineers can selectpolymers suitable for specific equipment as well as products.Polymer Mixing and Extrusion Technology examines viscometric techniquesand demonstrates their importance to product quality assurance ... reviews design-relatedliterature/correlations and calculation procedures for mixing and extrusion ... definesneeds and precision standards for setting up a polymer processing laboratory so thatproduct quality control can be implemented in physical testing and processing research.. . plus more.Illustrated with over 200 diagrams, tables, and photographs that facilitate readers'understanding of the processes, Polymer Mixing and Extrusion Technology isan authoritative source for plastics, polymer, and chemical engineers, manufacturers ofplastics processing equipment, and advanced undergraduate and graduate students in thesedisciplines.

Practical Guide to Infrared Microspectroscopy

Practical Guide to Infrared Microspectroscopy PDF

Author: Howard J. Humecki

Publisher: CRC Press

Published: 1995-01-23

Total Pages: 487

ISBN-13: 1482273306

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This work represents a sound introduction to the fundamental principles of infrared microspectroscopy (IMS). It describes how IMS is used to solve specific microanalytical problems in a variety of disciplines, including forensic analysis, art conservation, and geological, pharmaceutical and electronics research. The book discusses when and how to u

Methods of Preparation for Electron Microscopy

Methods of Preparation for Electron Microscopy PDF

Author: David G. Robinson

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 204

ISBN-13: 364248848X

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In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.

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