The DNA Replication-Repair Interface

The DNA Replication-Repair Interface PDF

Author:

Publisher: Academic Press

Published: 2021-11-12

Total Pages: 454

ISBN-13: 0323907342

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Replication-Coupled Repair, Volume 661 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including the Repair of replication-born DNA breaks by sister chromatid recombination, High resolution and high throughput DNA cyclization measurements to interrogate DNA bendability, A programmable detection method for genomic signatures: from disease diagnosis to genome editing, Characterization of the telomerase modulating activities of yeast DNA helicases, Eukaryotic DNA replication with purified budding yeast proteins, Single molecule studies of yeast Rad51 paralogs, Light activation and deactivation of Cas9 for DNA repair studies, and more. Other chapters explore MIDAS: Direct sequencing to map mitotic DNA synthesis and common fragile sites at high precision, Studying the DNA damage response in embryonic systems, GLASS-ChIP to map Mre11 cleavage sites in the human genome, New chemical biology approaches to trap reaction intermediates in living cells, Single-molecule imaging approaches for monitoring replication fork conflicts at genomic DNA G4 structures and R-loops in human cells, Monitoring the replication of structured DNA through heritable epigenetic change, Visualizing replication fork encounters with DNA interstrand crosslinks, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology series Includes the latest information on replication-coupled repair

Advances in DNA Repair

Advances in DNA Repair PDF

Author: Clark Chen

Publisher: BoD – Books on Demand

Published: 2015-11-18

Total Pages: 498

ISBN-13: 9535122096

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This book edition is intended to provide a concise summary for select topics in DNA repair, a field that is ever-expanding in complexity and biologic significance. The topics reviewed ranged from fundamental mechanisms of DNA repair to the interface between DNA repair and a spectrum on cellular process to the clinical relevance of DNA repair in oncologic paradigms. The information in this text should provide a foundation from which one can explore the various topics in depth. The book serve as a supplementary text in seminar courses with focus on DNA repair as well as a general reference for scholars with an interest in DNA repair.

Elucidating the Role of the Escherichia Coli RarA Protein at the Interface of DNA Replication and DNA Repair

Elucidating the Role of the Escherichia Coli RarA Protein at the Interface of DNA Replication and DNA Repair PDF

Author: Tyler H. Stanage

Publisher:

Published: 2017

Total Pages: 0

ISBN-13:

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In each cell cycle, the replisome encounters replication blocks that include DNA lesions, tightly bound DNA-protein complexes, and breaks in the DNA. In Escherichia coli, many pathways have evolved to recognize, respond to, and repair these replication blocks so that replication can be completed in a timely and accurate manner. The highly conserved RarA (Replication associated recombination protein A) protein family has been implicated in the faithful resolution of these replication conflicts. Despite high conservation and extensive work conducted on this protein family, the overall function of RarA remains enigmatic. Here, we carefully describe two novel DNA-dependent functions for the E. coli RarA protein. First, we demonstrated that RarA binds to and hydrolyzes ATP at double-stranded DNA ends in order to generate single-stranded DNA flaps. Second, using a single-molecule reconstituted DNA replication assay, we show that RarA generated single-stranded DNA gaps behind active replisomes in an ATP-dependent manner. This activity is achieved by RarA disengaging DNA polymerase III cores from their [beta]2 processivity clamps. In vivo, we demonstrate that RarA--presumably using its gap creation activity--generates suitable substrates for post-replication repair pathways including RecFOR-mediated homologous recombination and translesion DNA synthesis. Loss of this function results in impaired growth and varied DNA replication phenotypes. Overexpression of rarA is toxic due to its ATPase activity and the resulting overuse of mutagenic TLS polymerases. Together, these results further elucidate the role of the RarA protein family at the interface of DNA replication and DNA repair.

DNA Damage Recognition

DNA Damage Recognition PDF

Author: Wolfram Siede

Publisher: CRC Press

Published: 2005-09-19

Total Pages: 871

ISBN-13: 0849352681

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Stands as the most comprehensive guide to the subject-covering every essential topic related to DNA damage identification and repair. Covering a wide array of topics from bacteria to human cells, this book summarizes recent developments in DNA damage repair and recognition while providing timely reviews on the molecular mechanisms employe

Chemical Probes in Biology

Chemical Probes in Biology PDF

Author: Manfred P. Schneider

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 403

ISBN-13: 9400709587

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This NATO Advanced Study Institute (co-sponsored by FEBS and INTAS) under the title "Chemical Probes in Biology" was designed to summarize and disseminate recent expert knowledge regarding a deeper understanding ofbiological phenomena on a molecular level. Such scientific activities -frequently termed Bio-organic Chemistry or Chemical Biology are constituting a highly interdisciplinary branch of chemistry beyond the traditional ways in which chemists and biologists have been working in the past. Thus, on this occasion we were bringing together senior experts from the disciplines of Chemistry and Biology in order to amalgamate their diverse yet basically common interests in this area. Ultimate goal was - next to an exchange of information between the two scientific cultures - the communication ofexciting possibilities in interdisciplinary research to the young scientists present. The meeting was held in the Anargyros and Korgialenios School on the Island ofSpeteses, Greece from 18-30 August 2002. The ASI was attended by a total of 91 scholars from 23 different countries. A group of 27 speakers presented a series of 34 highly stimulating, informative and educational lectures covering a broad range of topics relevant to the general theme ofthis meeting: Science at the InteifaceofChemistry, Biology and Medicine. The lectures were complemented by a total of 89 posters presented by the young scholars and a series of short lectures derived thereof This was clearly one ofthe highlites of the meeting creating a lively atmosphere of interaction and intellectual creativity - typical phenomena for the whole meeting.

DNA Replication, Recombination, and Repair

DNA Replication, Recombination, and Repair PDF

Author: Fumio Hanaoka

Publisher: Springer

Published: 2016-01-22

Total Pages: 548

ISBN-13: 443155873X

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This book is a comprehensive review of the detailed molecular mechanisms of and functional crosstalk among the replication, recombination, and repair of DNA (collectively called the "3Rs") and the related processes, with special consciousness of their biological and clinical consequences. The 3Rs are fundamental molecular mechanisms for organisms to maintain and sometimes intentionally alter genetic information. DNA replication, recombination, and repair, individually, have been important subjects of molecular biology since its emergence, but we have recently become aware that the 3Rs are actually much more intimately related to one another than we used to realize. Furthermore, the 3R research fields have been growing even more interdisciplinary, with better understanding of molecular mechanisms underlying other important processes, such as chromosome structures and functions, cell cycle and checkpoints, transcriptional and epigenetic regulation, and so on. This book comprises 7 parts and 21 chapters: Part 1 (Chapters 1–3), DNA Replication; Part 2 (Chapters 4–6), DNA Recombination; Part 3 (Chapters 7–9), DNA Repair; Part 4 (Chapters 10–13), Genome Instability and Mutagenesis; Part 5 (Chapters 14–15), Chromosome Dynamics and Functions; Part 6 (Chapters 16–18), Cell Cycle and Checkpoints; Part 7 (Chapters 19–21), Interplay with Transcription and Epigenetic Regulation. This volume should attract the great interest of graduate students, postdoctoral fellows, and senior scientists in broad research fields of basic molecular biology, not only the core 3Rs, but also the various related fields (chromosome, cell cycle, transcription, epigenetics, and similar areas). Additionally, researchers in neurological sciences, developmental biology, immunology, evolutionary biology, and many other fields will find this book valuable.

Bloom Syndrome: New Insights for the Healthcare Professional: 2012 Edition

Bloom Syndrome: New Insights for the Healthcare Professional: 2012 Edition PDF

Author:

Publisher: ScholarlyEditions

Published: 2012-12-10

Total Pages: 21

ISBN-13: 1464984476

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Bloom Syndrome: New Insights for the Healthcare Professional / 2012 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Bloom Syndrome in a compact format. The editors have built Bloom Syndrome: New Insights for the Healthcare Professional / 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Bloom Syndrome in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Bloom Syndrome: New Insights for the Healthcare Professional / 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

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