Genome Engineering via CRISPR-Cas9 System

Genome Engineering via CRISPR-Cas9 System PDF

Author: Vijai Singh

Publisher: Academic Press

Published: 2020-02-21

Total Pages: 0

ISBN-13: 9780128181409

DOWNLOAD EBOOK →

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.

Genome Engineering via CRISPR-Cas9 System

Genome Engineering via CRISPR-Cas9 System PDF

Author: Vijai Singh

Publisher: Academic Press

Published: 2020-02-18

Total Pages: 386

ISBN-13: 0128181419

DOWNLOAD EBOOK →

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields. Provides basic understanding and a clear picture on how to design, use and implement the CRISPR-Cas9 system in different organisms Explains how to create an animal model for disease research and screening purposes using CRISPR Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, neurology, cancer, industry, and many more

The CRISPR/Cas9 System

The CRISPR/Cas9 System PDF

Author: Alfred A. Bertelsen

Publisher:

Published: 2019

Total Pages: 0

ISBN-13: 9781536164268

DOWNLOAD EBOOK →

This compilation focuses on the CRISPR/Cas9 system, a genome editing tool that has been hailed as the most profound molecular biology discovery in the past decade. By employing the natural process of bacterial immunity towards bacteriophages, the tool allows researchers to precisely excise and edit parts of the genetic sequence to modify them.The authors summarize the molecular pathogenesis of hepatocellular carcinoma, available treatments/drugs and their limitations, the landscape of CRISPR targeting hepatocellular carcinoma, limitations and potential targets in future.The closing review summarises the use of CRISPR/Cas9 gene editing in ophthalmology and focuses on the advancement of gene editing in the cornea. The majority of corneal dystrophies are the result an autosomal dominant inheritance pattern within the TGFBI gene, which presents an ideal model suited for a CRISPR/Cas9 knock out methodology.

CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases

CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases PDF

Author: Luis María Vaschetto

Publisher: CRC Press

Published: 2022-01-31

Total Pages: 268

ISBN-13: 1000540812

DOWNLOAD EBOOK →

The CRISPR-Cas9 genome-editing system is creating a revolution in the science world. In the laboratory, CRISPR-Cas9 can efficiently be used to target specific genes, correct mutations and regulate gene expression of a wide array of cells and organisms, including human cells. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases is a unique reading material for college students, academicians, and other health professionals interested in learning about the broad range of applications of CRISPR/Cas9 genetic scissors. Some topics included in this book are: the role of the CRISPR/Cas9 system in neuroscience, gene therapy, epigenome editing, genome mapping, cancer, virus infection control strategies, regulatory challenges and bioethical considerations.

CRISPR-Cas Systems

CRISPR-Cas Systems PDF

Author: Rodolphe Barrangou

Publisher: Springer Science & Business Media

Published: 2012-12-13

Total Pages: 300

ISBN-13: 364234657X

DOWNLOAD EBOOK →

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

Plant Genome Editing with CRISPR Systems

Plant Genome Editing with CRISPR Systems PDF

Author: Yiping Qi

Publisher: Springer Nature

Published: 2019-01-04

Total Pages: 360

ISBN-13: 149398991X

DOWNLOAD EBOOK →

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

A Crack In Creation

A Crack In Creation PDF

Author: Jennifer A. Doudna

Publisher: HarperCollins

Published: 2017-06-13

Total Pages: 307

ISBN-13: 0544716965

DOWNLOAD EBOOK →

BY THE WINNER OF THE 2020 NOBEL PRIZE IN CHEMISTRY | Finalist for the Los Angeles Times Book Prize “A powerful mix of science and ethics . . . This book is required reading for every concerned citizen—the material it covers should be discussed in schools, colleges, and universities throughout the country.”— New York Review of Books Not since the atomic bomb has a technology so alarmed its inventors that they warned the world about its use. That is, until 2015, when biologist Jennifer Doudna called for a worldwide moratorium on the use of the gene-editing tool CRISPR—a revolutionary new technology that she helped create—to make heritable changes in human embryos. The cheapest, simplest, most effective way of manipulating DNA ever known, CRISPR may well give us the cure to HIV, genetic diseases, and some cancers. Yet even the tiniest changes to DNA could have myriad, unforeseeable consequences, to say nothing of the ethical and societal repercussions of intentionally mutating embryos to create “better” humans. Writing with fellow researcher Sam Sternberg, Doudna—who has since won the Nobel Prize for her CRISPR research—shares the thrilling story of her discovery and describes the enormous responsibility that comes with the power to rewrite the code of life. “The future is in our hands as never before, and this book explains the stakes like no other.” — George Lucas “An invaluable account . . . We owe Doudna several times over.” — Guardian

CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases

CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases PDF

Author: Luis María Vaschetto

Publisher: CRC Press

Published: 2022-01-31

Total Pages: 315

ISBN-13: 1000540839

DOWNLOAD EBOOK →

The CRISPR-Cas9 genome-editing system is creating a revolution in the science world. In the laboratory, CRISPR-Cas9 can efficiently be used to target specific genes, correct mutations and regulate gene expression of a wide array of cells and organisms, including human cells. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases is a unique reading material for college students, academicians, and other health professionals interested in learning about the broad range of applications of CRISPR/Cas9 genetic scissors. Some topics included in this book are: the role of the CRISPR/Cas9 system in neuroscience, gene therapy, epigenome editing, genome mapping, cancer, virus infection control strategies, regulatory challenges and bioethical considerations.

CRISPR-Cas

CRISPR-Cas PDF

Author: Jennifer A. Doudna

Publisher:

Published: 2016

Total Pages: 0

ISBN-13: 9781621821304

DOWNLOAD EBOOK →

CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.

Targeted Genome Editing Using Site-Specific Nucleases

Targeted Genome Editing Using Site-Specific Nucleases PDF

Author: Takashi Yamamoto

Publisher: Springer

Published: 2015-01-05

Total Pages: 206

ISBN-13: 4431552278

DOWNLOAD EBOOK →

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.