Principles of Analytical Biochemistry

Principles of Analytical Biochemistry PDF

Author: Thomas Rutherford

Publisher:

Published: 2019-08-23

Total Pages:

ISBN-13: 9781645320296

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Analytical techniques such as spectroscopy, chromatography, etc. are particularly important in analytical biochemistry as well as in analytical chemistry generally. The principles of each technique are explained and the scope and applications are discussed. There are chapters on enzymes, antibodies and radio-isotopes, substances which it may be necessary to detect and measure but which also can be very useful in a variety of analytical methods. The present title "Principles of Analytical Biochemistry" is intended for those who wish to understand living organisms, especially man. Biochemistry is essential for this purpose, but it would be almost impossible for a student to survey on his own the massive body of existing knowledge, constantly augmented by a remarkable torrent of brilliant discoveries. Biochemistry is the study of the chemistry of living things. This includes organic molecules and their chemical reactions. Most people consider biochemistry to be synonymous with molecular biology. Biochemistry today has made spectacular progress in unraveling the mysteries of animate nature. This progress has allowed us to gain deeper insight into the principles of vital activity and has to a very significant extent stimulated the development of applied disciplines, especially medicine. The aim of this book is to present a core of biochemical knowledge that is desirable for undergraduate and postgraduate students and also those involved in the field of medical, microbiology, biotechnology and pharmaceutical.

Bioanalytics

Bioanalytics PDF

Author: Friedrich Lottspeich

Publisher: John Wiley & Sons

Published: 2018-03-08

Total Pages: 450

ISBN-13: 3527694447

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Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Biochemical Methods of Analysis

Biochemical Methods of Analysis PDF

Author: Saroj Dua

Publisher: Alpha Science International, Limited

Published: 2010

Total Pages: 228

ISBN-13:

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A comprehensive discussion of the various analytical techniques that are carried out in biochemistry, intends to support students in grasping techniques which are of use for theoretical and practical purposes.

Analytical Techniques in Biochemistry and Molecular Biology

Analytical Techniques in Biochemistry and Molecular Biology PDF

Author: Rajan Katoch

Publisher: Springer Science & Business Media

Published: 2011-07-19

Total Pages: 449

ISBN-13: 1441997857

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Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology PDF

Author: Andreas Hofmann

Publisher: Cambridge University Press

Published: 2018-04-19

Total Pages: 962

ISBN-13: 1108365256

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Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.

Analytical Techniques in Biosciences

Analytical Techniques in Biosciences PDF

Author: Chukwuebuka Egbuna

Publisher: Academic Press

Published: 2021-10-21

Total Pages: 302

ISBN-13: 0128227990

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Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry. The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences. Presents basic analytical protocols and sample-preparation guidelines Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research Presents biostatistical tools and methods and basic computational models in biosciences