Practical Approach to Mammalian Cell and Organ Culture

Practical Approach to Mammalian Cell and Organ Culture PDF

Author: Tapan Kumar Mukherjee

Publisher: Springer Nature

Published: 2022-11-07

Total Pages: 1286

ISBN-13: 9811917310

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This Major Reference Work offers a detailed overview of culturing primary, secondary cell lines, tissues, and organs. It first introduces various types of mammalian cell cultures, infrastructure requirements for a mammalian cell-culture laboratory. The subsequent chapters present the detailed protocols for the isolation of mammalian hematologic organs and cells. It also discusses various cell-based assays for monitoring cell viability, cell proliferation, cytotoxicity, cell senescence, and cell death assays. In addition, the book addresses the various problems encountered while culturing animal cells, their possible causes, and suggested solutions, presenting detailed protocols for isolation and primary culturing of various mammalian cells and hematoimmunologic organs in two dimensions. Lastly, it reviews the various applications of animal-cell culture, stem-cell culture, and tissue and organ culture. As such, this reference book is highly relevant for students and professionals new to cell-culture work as well as to those wishing to expand their skills from cell-line cultures to primary cultures and from conventional 2D cultures to 3D cultures.

Animal Cell Culture

Animal Cell Culture PDF

Author: R. Ian Freshney

Publisher: Oxford University Press, USA

Published: 1992

Total Pages: 358

ISBN-13:

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Animal Cell Culture: A Practical Approach has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the series. This new edition takes account of the progress that has been made in the field. Although the basic principles remain the same, significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetric indicator of viability has made a considerable impact on cytotoxity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presenting techniques in a readily accessible form, with detailed protocols given throughout. This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.

Plant Cell Culture

Plant Cell Culture PDF

Author: R. A. Dixon

Publisher: IRL Press

Published: 1994

Total Pages: 254

ISBN-13: 9780199634026

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Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad readership, that made the first edition so successful. Internationally recognized experts describe the techniques used for isolating and manipulating cell cultures, and the central importance in plant biotechnology. The book will be of major interest to researchers in plant sciences in general, and specifically to botany, plant physiology, and biotechnology students.

Basic Cell Culture

Basic Cell Culture PDF

Author: John M. Davis

Publisher: Oxford University Press, USA

Published: 1994

Total Pages: 336

ISBN-13:

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In the last 20 years cell culture has developed enormously from being used only in specialized areas of research to being the cornerstone of probably the world's fastest-growing industry, biotechnology. The primary aim of this book is to guide the newcomer progressively through all those areas which nowadays are fundamental to the performance of cell culture. The book will also prove useful to the experienced worker. Topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, culture technique, the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination, and good laboratory practice in the cell culture laboratory.

Mammalian Cell Biotechnology

Mammalian Cell Biotechnology PDF

Author: Michael Butler

Publisher: Oxford University Press

Published: 1991

Total Pages: 245

ISBN-13: 9780199632091

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The development of mammalian cell biotechnology has led to an extensive range of compounds which can be routinely produced by cell culture. This book details the principles and practical techniques upon which this development is based.

A Beginner's Guide to Mammalian Cell Culture

A Beginner's Guide to Mammalian Cell Culture PDF

Author: Jayakody Pathirannehelage Tharindunee Jayakody

Publisher: Blue Rose Publishers

Published: 2022-07-07

Total Pages: 58

ISBN-13:

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A Beginners Guide to Mammalian Cell Culture contains the basics and the most essential knowledge on culturing mammalian cells useful for any researcher to start mammalian cell culture independently. It’s written in a simple language and is equipped with images of cell cultures and illustrations for more clarity. The contents of the book include guidance for researchers to choose appropriate cell lines, reagents, equipment and consumables for their research, usage and maintenance of equipment, basic information on bio-safety levels and precautions that must be taken at each level to ensure safety of the personnel handling cell cultures, microbial contaminations and how to avoid them and most importantly tips to maintain cell cultures and to trouble shoot issues independently. The book has been edited by Dr. Francis Tan, a senior research fellow at the Department of Anesthesia, National University of Singapore. Dr. Tan has many years of experience in culturing mammalian cells, maintaining laboratory equipment and training researchers in these techniques, which has been a valuable source of input for the completion of this book.

Introduction to Cell and Tissue Culture

Introduction to Cell and Tissue Culture PDF

Author: Jennie P. Mather

Publisher: Springer Science & Business Media

Published: 2007-08-20

Total Pages: 247

ISBN-13: 0585275718

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It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

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