A Practical Guide to Optical Microscopy

A Practical Guide to Optical Microscopy PDF

Author: John Girkin

Publisher: CRC Press

Published: 2019-06-14

Total Pages: 247

ISBN-13: 1351630350

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Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

Basic Methods in Microscopy

Basic Methods in Microscopy PDF

Author: David L. Spector

Publisher: CSHL Press

Published: 2006

Total Pages: 384

ISBN-13: 0879697512

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This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.

Light Microscopy in Biology

Light Microscopy in Biology PDF

Author: Alan J. Lacey

Publisher: OUP Oxford

Published: 1999-06-03

Total Pages: 475

ISBN-13: 0191565725

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Since the first edition of Light Microscopy in Biology: A Practical approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that have been developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. Totally new topics covered include: confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. There are also whole chapters now devoted to reflection contrast microscopy and histomorphometry. This new edition will be of great interest to postgraduate and postdoctoral researchers in biomedicine and cell biology - both those experienced with light microscopic techniques and newcomers to the field.

Diagnostic Electron Microscopy

Diagnostic Electron Microscopy PDF

Author: John Stirling

Publisher: John Wiley & Sons

Published: 2013-01-22

Total Pages: 492

ISBN-13: 1119973996

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Diagnostic Electron Microscopy Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.

Quantitative Imaging in Cell Biology

Quantitative Imaging in Cell Biology PDF

Author:

Publisher: Academic Press

Published: 2014-06-25

Total Pages: 609

ISBN-13: 0124202012

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This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Practical Methods in Microscopy (Classic Reprint)

Practical Methods in Microscopy (Classic Reprint) PDF

Author: Charles H. Clark

Publisher: Forgotten Books

Published: 2017-11-28

Total Pages: 292

ISBN-13: 9780332146539

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Excerpt from Practical Methods in Microscopy This book, as originally written, was intended for begin ners working privately without the assistance of experienced instructors and without the apparatus Of a well-equipped laboratory. It was, therefore, thought best not to include processes which require expensive accessory apparatus and a considerable outfit of chemicals. The complicated processes necessary for the best results with delicate tissues were also Omitted as beyond the needs of the mere beginner and as likely to discourage him in his first efforts. The favor with which the book has been received in the laboratories of several leading colleges has led to the addi tions which have been made in this edition to include the processes Of microscopic technique that are needed in such laboratories. While the book has been extended to meet the needs Of more advanced workers, it is believed that it is none the less adapted to the needs of beginners and private workers. The author wishes to make especial acknowledgment Of indebtedness for suggestions to Professor Frederick C. New combe, Of the University of Michigan, and to Professor F. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.

Practical Electron Microscopy

Practical Electron Microscopy PDF

Author: Elaine Evelyn Hunter

Publisher: Cambridge University Press

Published: 1993-09-24

Total Pages: 210

ISBN-13: 9780521385398

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For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added--on immuno electron microscopy using colloidal gold and on useful specialized techniques.