Plant Transposable Elements

Plant Transposable Elements PDF

Author: Marie-Angèle Grandbastien

Publisher: Springer Science & Business Media

Published: 2013-01-08

Total Pages: 338

ISBN-13: 3642318428

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Transposable elements are short lengths of DNA with the capacity to move between different points within a genome. This process can affect the function of genes at or near the insertion site. The present book gives an overview of the impact of transposable elements on plant genomes and explains how to recognize and study transposable elements, e.g. by using state-of-the-art strategies like “new generation sequencing.” Moreover, the impact of transposable elements on plant genome structure and function is reviewed in detail, and also illustrated in examples and case studies. The book is intended both for readers familiar with the field and for newcomers. With large-scale sequencing becoming increasingly available, more and more people will come across transposable element sequences in their data, and this volume will hopefully help to convince them that they are not just “junk DNA.”

Plant Transposable Elements

Plant Transposable Elements PDF

Author: Jungnam Cho

Publisher: Humana

Published: 2022-05-11

Total Pages: 272

ISBN-13: 9781071611364

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This volume details the most up-to-date technologies used in plant transposable element studies and provides easy-to-follow protocols. Chapters guide readers on available database resources, annotation of different families of transposon, and experimental methods to detect their transposition intermediates, neo-transposed DNAs, and transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Transposable Elements: Methods and Protocols aims to provide web-lab and dry-lab methodologies targeted at various levels from beginner to experienced.

Plant Transposable Elements

Plant Transposable Elements PDF

Author: Deepu Pandita

Publisher:

Published: 2023

Total Pages: 0

ISBN-13: 9781774911822

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"This new volume provides an up-to-date understanding of the numerous classes of plant transposable elements, the mobile units of DNA that comprise large portions of plant genomes, an important contributor for gene and genome evolution. Transposable elements (TEs) are major components of large plant genomes and main drivers of genome evolution, known to produce a wide variety of changes in plant gene expression and function. This book, Plant Transposable Elements: Biology and Biotechnology, provides a systematic interpretation of protocols designed to characterize TEs and their biotechnological roles. The chapters explore the role of TEs in plant development, their architecture, their epigenetic regulation, their use in DNA repair, their evolution and speciation, while highlighting their importance in the approaching epoch of climate change. It discusses the applications of the transposons in genome editing and their biology, classification, structure, functions, and evolution. The volume begins with introduction of transposable elements (TEs), covering their introduction, classification, and transposition. It delves into protocols designed to characterize TEs and their biotechnological applications. This section looks at computational approaches for prediction and analysis, retro-transposon capture sequencing, and more. The section on transposon biology focuses on its role in plant development and as natural genetic engineers of genome mutation, evolution, and speciation. The book looks further into transposon applications in genome editing, exploring tagging and mutagenesis, genome engineering, and more. The last chapter uses the example of Oryza sativa to elucidate on the classification, structure, function, and evolution of transposable elements. This comprehensive volume is a valuable comprehensive resource for researchers, faculty, and students in biology, biotechnology, genetics, and botany and plant science."--

Plant Transposable Elements

Plant Transposable Elements PDF

Author: O. Nelson

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 396

ISBN-13: 1468455508

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Transposon tagging can work. Even though most of our understand ing about the factors that contribute to a successful tagging experiment has been accumulated from a limited number of experiments using different transposable elements in different genetic backgrounds, it is still possible to draw some conclusions regarding the best experimental strategies for gene tagging. In our experience, Spm has proved to be a good element for transposon tagging. The frequency of recovering mutable alleles in duced by Spm is not significantly different from that for Ac-Ds or for Mu 6 (summarized in Ref. 22) and varies from about 10- to 10=zr:-8pm has the unique advantage, however, in that all of the members of thiSfumily that have been examined thus far are homologous to each other at the DNA level. Therefore, by combining molecular analysis with genetic segre gation, it is possible to identify and isolate alleles that are due to insertions of either autonomous or nonautonomous Spm elements. There are definite steps one can take to increase the chances of de tecting a transposition into the gene of interest. The most important step is to select a genetic background in which the desired phenotype will be easy to screen. If the phenotype is not likely to be mutable, then tester lines should be constructed so as to contain flanking markers that can aid in subsequent segregation analyses.

Plant Transposable Elements

Plant Transposable Elements PDF

Author: Jungnam Cho

Publisher:

Published: 2021

Total Pages: 272

ISBN-13: 9781071611340

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This volume details the most up-to-date technologies used in plant transposable element studies and provides easy-to-follow protocols. Chapters guide readers on available database resources, annotation of different families of transposon, and experimental methods to detect their transposition intermediates, neo-transposed DNAs, and transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Transposable Elements: Methods and Protocols aims to provide web-lab and dry-lab methodologies targeted at various levels from beginner to experienced.

Plant Genome Diversity Volume 1

Plant Genome Diversity Volume 1 PDF

Author: Jonathan Wendel

Publisher: Springer Science & Business Media

Published: 2012-03-23

Total Pages: 282

ISBN-13: 3709111307

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In this timely new 2-volume treatise, experts from around the world have banded together to produce a first-of-its-kind synopsis of the exciting and fast moving field of plant evolutionary genomics. In Volume I of Plant Genome Diversity, an update is provided on what we have learned from plant genome sequencing projects. This is followed by more focused chapters on the various genomic “residents” of plant genomes, including transposable elements, centromeres, small RNAs, and the evolutionary dynamics of genes and non-coding sequences. Attention is drawn to advances in our understanding of plant mitochondrial and plastid genomes, as well as the significance of duplication in genic evolution and the non-independent evolution among sequences in plant genomes. Finally, Volume I provides an introduction to the vibrant new frontier of plant epigenomics, describing the current state of our knowledge and the evolutionary implications of the epigenomic landscape.

The Maize Genome

The Maize Genome PDF

Author: Jeffrey Bennetzen

Publisher: Springer

Published: 2018-11-24

Total Pages: 390

ISBN-13: 3319974270

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This book discusses advances in our understanding of the structure and function of the maize genome since publication of the original B73 reference genome in 2009, and the progress in translating this knowledge into basic biology and trait improvement. Maize is an extremely important crop, providing a large proportion of the world’s human caloric intake and animal feed, and serving as a model species for basic and applied research. The exceptionally high level of genetic diversity within maize presents opportunities and challenges in all aspects of maize genetics, from sequencing and genotyping to linking genotypes to phenotypes. Topics covered in this timely book range from (i) genome sequencing and genotyping techniques, (ii) genome features such as centromeres and epigenetic regulation, (iii) tools and resources available for trait genomics, to (iv) applications of allele mining and genomics-assisted breeding. This book is a valuable resource for researchers and students interested in maize genetics and genomics.

Plant Transposable Elements

Plant Transposable Elements PDF

Author: Marie-Angèle Grandbastien

Publisher: Springer

Published: 2013-01-08

Total Pages: 330

ISBN-13: 9783642318436

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Transposable elements are short lengths of DNA with the capacity to move between different points within a genome. This process can affect the function of genes at or near the insertion site. The present book gives an overview of the impact of transposable elements on plant genomes and explains how to recognize and study transposable elements, e.g. by using state-of-the-art strategies like “new generation sequencing.” Moreover, the impact of transposable elements on plant genome structure and function is reviewed in detail, and also illustrated in examples and case studies. The book is intended both for readers familiar with the field and for newcomers. With large-scale sequencing becoming increasingly available, more and more people will come across transposable element sequences in their data, and this volume will hopefully help to convince them that they are not just “junk DNA.”

Transposable Elements in Plants

Transposable Elements in Plants PDF

Author: Andrew Kalinski

Publisher: DIANE Publishing

Published: 1995-07

Total Pages: 194

ISBN-13: 9780788119897

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The following terms were used in this search: plant/s, transposable element/s, and mobile element/s. Includes the following information for the sponsored projects: title, investigators, performing organization, location of performing organization, objectives, and approach. The bibliography includes 129 citations from 1984 through 1991, and 162 citations 1992 through Sep. 1994. Some citations have abstracts.

10 Years Plant Molecular Biology

10 Years Plant Molecular Biology PDF

Author: R.A. Schilperoort

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 199

ISBN-13: 9401126569

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It is very clear nowadays that plants offer several opportunities for basic studies, e.g. on development and embryogenesis, and that the fundamental principles laid open contribute to the development of new tools for plant breeding. Within the scope of the present publication, the editors have had to make a difficult choice from the many important subjects that have contributed to the remarkable progress of our molecular biological understanding of complex biological problems. This has resulted in review papers showing the present state of the art in genetic engineering, gene expression and its manipulation, microbe and insect interactions with plants, transposable elements and gene tagging, plant and organ development, the function and structure of the genome chloroplasts, and lipid biosynthesis. All papers have been written in such a way that they are also useful for non-experts interested in a particular field, as well as for students following courses in plant molecular biology. Besides presenting the state of the art, each paper gives some historical background to the developments in the field as well as perspectives for further basic research and applications. Because of the latter, scientists and students engaged in plant breeding will also profit from this publication.