Plant DNA Infectious Agents

Plant DNA Infectious Agents PDF

Author: Thomas Hohn

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 355

ISBN-13: 3709169771

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There has been recent rapid progress in the transformation of plants with foreign DNA, making use either of the natural routes of genetic invasion that viruses and bacteria have developed, or of chemical, mechanical and electrical tricks to make plant protoplast membranes permeable to nucleic acids. Genes integrated into plant virus genomes can be carried systemi cally from the initial site of infection into the rest of the plant. Genes placed between the borders of Agrobacterium tumefaciens T-DNA can be transferred into single cells or plant tissue, which then divides to produce wound calli, or as in the case of an Agrobacterium rhizogenes infection, grow out into new roots. Calli and roots can be grown into whole plants. If virus genomes are placed between the T-DNA borders, a very effective infectious route, termed "agroinfection", is established. Once inside a pro toplast, DNA finds its way into the nucleus where it can finally integrate into the resident chromosome and be expressed. Whether it can also find its way into chloroplasts is not yet clear, but at least translation products can be targeted into this organelle. Regeneration of whole organisms from single cells is a special feature of plants and offers a unique tool to study genes in a multicellular organism. In addition, as in animal cells, transcription and translation of trans forming genes can be studied in plant cells during "transient expression".

Biosafety in the Laboratory

Biosafety in the Laboratory PDF

Author: Division on Engineering and Physical Sciences

Publisher: National Academies Press

Published: 1989-01-01

Total Pages: 237

ISBN-13: 0309039754

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Biosafety in the Laboratory is a concise set of practical guidelines for handling and disposing of biohazardous material. The consensus of top experts in laboratory safety, this volume provides the information needed for immediate improvement of safety practices. It discusses high- and low-risk biological agents (including the highest-risk materials handled in labs today), presents the "seven basic rules of biosafety," addresses special issues such as the shipping of dangerous materials, covers waste disposal in detail, offers a checklist for administering laboratory safetyâ€"and more.

The Epstein-Barr Virus

The Epstein-Barr Virus PDF

Author: M. A. Epstein

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 467

ISBN-13: 3642672361

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The Epstein-Barr virus was discovered 15 years ago. Since that time an immense body of information has been accumu lated on this agent which has come to assume great signifi cance in many different fields of biological science. Thus, the virus has very special relevance in human medicine and oncology, in tumor virology, in immunology, and in mole cular virology, since it is the cause of infectious mononu cleosis and also the first human cancer virus, etiologically related to endemic Burkitt's lymphoma and probably to nasopharyngeal carcinoma. In addition, continuous human lymphoid cell lines initiated and maintained by the transform ing function of the virus genome provide a laboratory tool with wide and ever-growing applications. Innumerable papers on the Epstein-Barr virus have ap peared over recent years and reports of work with this agent now constitute a veritable flood. The present book provides the first and only comprehensive, authoritative over-view of all aspects of the virus by authors who have been the original and major contributors in their particular disciplines. A complete and up-to-date survey of this unique and important agent is thus provided which should be of great interest to experts, teachers, and students engaged in cancer research, virology, immunology, molecular biology, epide miology, and cell culture. Where topics have been dealt with from more than one of these viewpoints, some inevitable overlap and duplication has resulted; although this has been kept to a minimum, it has been retained in some places because of positive usefulness.

Responsible Research with Biological Select Agents and Toxins

Responsible Research with Biological Select Agents and Toxins PDF

Author: National Research Council

Publisher: National Academies Press

Published: 2010-02-12

Total Pages: 188

ISBN-13: 030914535X

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The effort to understand and combat infectious diseases has, during the centuries, produced many key advances in science and medicine-including the development of vaccines, drugs, and other treatments. A subset of this research is conducted with agents that, like anthrax, not only pose a severe threat to the health of humans, plants, and animals but can also be used for ill-intended purposes. Such agents have been listed by the government as biological select agents and toxins. The 2001 anthrax letter attacks prompted the creation of new regulations aimed at increasing security for research with dangerous pathogens. The outcome of the anthrax letter investigation has raised concern about whether these measures are adequate. Responsible Research with Biological Select Agents and Toxins evaluates both the physical security of select agent laboratories and personnel reliability measures designed to ensure the trustworthiness of those with access to biological select agents and toxins. The book offers a set of guiding principles and recommended changes to minimize security risk and facilitate the productivity of research. The book recommends fostering a culture of trust and responsibility in the laboratory, engaging the community in oversight of the Select Agent Program, and enhancing the operation of the Select Agent Program.

Virus Structure

Virus Structure PDF

Author:

Publisher: Elsevier

Published: 2003-10-02

Total Pages: 610

ISBN-13: 0080493777

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Virus Structure covers the full spectrum of modern structural virology. Its goal is to describe the means for defining moderate to high resolution structures and the basic principles that have emerged from these studies. Among the topics covered are Hybrid Vigor, Structural Folds of Viral Proteins, Virus Particle Dynamics, Viral Gemone Organization, Enveloped Viruses and Large Viruses. Covers viral assembly using heterologous expression systems and cell extracts Discusses molecular mechanisms in bacteriophage T7 procapsid assembly, maturation and DNA containment Includes information on structural studies on antibody/virus complexes

Plant Molecular Biology 2

Plant Molecular Biology 2 PDF

Author: R.G. Herrmann

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 747

ISBN-13: 146153304X

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The VI NATO Advanced Study Institute on Plant Molecular Biology, held in Elmau, Bavaria, Germany, from 14 to 23 May, 1990, brought together representative scientific leaders from all over the world to review their lastest results. They presented lectures or posters, participated in lively discussions, educated students, and exchanged views and plans for future research in this highly exciting field of science. The experiments, data and questions were naturally varied, but all of them illustrate that the modern techniques of molecular biology, complemented by developments in immunology, genetics, and ultrastructural research, have pervaded nearly every branch of biology. The presentations show that these approaches have tremendously increased our potential both for fundamental research, our understanding of life, and by analogy to the precedents of physics and chemistry, have led and will continue to lead to "engineering sciences" and implicitly, to new industrial processes. Some of these applications are a matter of debate in the public domain today and many feel that the development of industrial gene technology requires the attention of the whole scientific community. Nevertheless, the implications of this research for the genetic improvement of agricultural plants are profound. Some of the near term technologies being developed provide novel approaches for improving the utility of food crops. They can also result in reduced dependence on the use of pesticides for food production.

Viral Genes and Plant Pathogenesis

Viral Genes and Plant Pathogenesis PDF

Author: Thomas P. Pirone

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 229

ISBN-13: 1461234247

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The chapters in this book represent detailed versions of papers presented at the Symposium on Viral Genes and Plant Pathogenesis held at Lexington, Kentucky on October 16 and 17, 1989. In selecting topics and authors, we attempted to have represented a spectrum of systems which are at the forefront of research on plant virus genes and gene products, particularly as they relate to plant disease. The book also contains pertinent discussion of the papers presented at the symposium, as well as summaries, observations and projections of future research directions prepared by the session chairmen. We wish to express our appreciation to Dr. D. L. Davis, of the RI. Reynolds Tobacco Company for suggesting the organization of the Symposium and the publication of the proceedings, and to the R 1. Reynolds Tobacco Company for the financial support which made the symposium possible. We also wish to thank those of our colleagues in the Department of Plant Pathology, University of Kentucky, who very ably and in many ways contributed to the organization and conduct of the conference. Thomas P. Pirone lohnG. Shaw v Contents Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi INDUCTION OF HOST GENES BY THE HYPERSENSITIVE RESPONSE OF TOBACCO TO VIRUS INFECTION J. F. Bol, C. M. A. van Rossum, Bl. C. Cornelissen and H. J. M. Linthorst . . . . . . . . . . . . . . . . . l COAT PROTEIN MEDIATED RESISTANCE IN TRANSGENIC PLANTS Roger N. Beachy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 STRUCTURE AND FUNCTIONS OF TOBACCO MOSAIC VIRUS RNA Yoshimi Okada, Tetsuo Meshi, and Yuichiro Watanabe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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