-PDF Download- Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 236 Fish Embryo Acute Toxicity Fet Test EBOOK

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 236: Fish Embryo Acute Toxicity (FET) Test

Author: OECD

Publisher: OECD Publishing

Published: 2013-07-26

Total Pages: 22

ISBN-13: 9264203702

The test method described in this Test Guidelineis inteneded to determine the acute or letal toxicity of chemicals on embryonic stages of fish (Danio rerio). Newly fertilised zebrafish eggs are exposed to the test chemical for a period of 96 hrs ...

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 203: Fish, Acute Toxicity Test

Author: OECD

Publisher: OECD Publishing

Published: 2019-06-18

Total Pages: 24

ISBN-13: 9264069968

DOWNLOAD EBOOK →

The fish are exposed to the test substance preferably for a period of 96 hours. Mortalities are recorded at 24, 48, 72 and 96 hours and the concentrations which kill 50 per cent of the fish (LC50) are determined where possible. One or more species ...

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 210: Fish, Early-life Stage Toxicity Test

Author: OECD

Publisher: OECD Publishing

Published: 2013-07-26

Total Pages: 24

ISBN-13: 9264203788

DOWNLOAD EBOOK →

The test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species tested. The early-life stages of fish are exposed to five concentrations of the test ...

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages

Author: OECD

Publisher: OECD Publishing

Published: 1998-09-21

Total Pages: 20

ISBN-13: 9264070141

DOWNLOAD EBOOK →

In this Short-term Toxicity Test on Fish Embryo and Sac-Fry Stages, the life stages from the newly fertilized egg to the end of the sac-fry stage are exposed. The embryo and sac-fry stages of fish are exposed to five concentrations of the test ...

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 204: Fish, Prolonged Toxicity Test: 14-Day Study

Author: OECD

Publisher: OECD Publishing

Published: 1984-04-04

Total Pages: 9

ISBN-13: 9264069984

DOWNLOAD EBOOK →

This Test Guideline presents guidance for measurement of lethal and other observed effects in fish exposed to test substances. It may be used in place of Test Guideline 203 if a longer observation period is considered useful and appropriate and the ...

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay

Author: OECD

Publisher: OECD Publishing

Published: 2021-06-18

Total Pages: 51

ISBN-13: 9264714138

DOWNLOAD EBOOK →

The RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed based on three fluorescent cell viability indicator dyes, measured on the same set of cells. Resazurin enters the cells in its non-fluorescent form and is converted to the fluorescent product, resorufin, by mitochondrial, microsomal or cytoplasmic oxidoreductases.

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 215: Fish, Juvenile Growth Test

Author: OECD

Publisher: OECD Publishing

Published: 2000-01-21

Total Pages: 16

ISBN-13: 9264070206

DOWNLOAD EBOOK →

This Test Guideline is designed to assess the effects of prolonged exposure to chemicals on the growth of juvenile fish. Juvenile fish in exponential growth phase are placed, after being weighed, in test chambers and are normally exposed to five ...

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 223: Avian Acute Oral Toxicity Test

Author: OECD

Publisher: OECD Publishing

Published: 2016-07-29

Total Pages: 28

ISBN-13: 9264264515

DOWNLOAD EBOOK →

This Test Guideline describes procedures designed to estimate the acute oral toxicity of substances to birds, and it provides three testing options: (1) limit dose test, (2) LD50-slope test, and (3) LD50-only test. The LD50-slope and LD50-only options are sequential testing procedures.

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 240: Medaka Extended One Generation Reproduction Test (MEOGRT)

Author: OECD

Publisher: OECD Publishing

Published: 2023-07-04

Total Pages: 53

ISBN-13: 9264242252

DOWNLOAD EBOOK →

This Test Guideline describes the Medaka Extended One Generation Test (MEOGRT), which exposes fish over multiple generations to give data relevant to ecological hazard and risk assessment of chemicals, including suspected endocrine disrupting chemicals (EDCs). Exposure in the MEOGRT starts with ...

OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 250: EASZY assay - Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos

Author: OECD

Publisher: OECD Publishing

Published: 2021-06-17

Total Pages: 45

ISBN-13: 9264886109

DOWNLOAD EBOOK →

The EASZY assay is a mechanism-based in vivo screening assay designed to detect endocrine active chemicals acting as agonist through estrogen receptors (ERs), by inducing the expression of the green fluorescent protein (GFP) driven by the cyp19a1b promoter. The EASZY assay allows for the detection of estrogenic activity of chemicals on transgenic tg(cyp19a1b:GFP) Zebrafish embryos exposed for 96 hours during the embryonic stages of development.