Gel Electrophoresis of Nucleic Acids

Gel Electrophoresis of Nucleic Acids PDF

Author: David Rickwood

Publisher: Oxford University Press, USA

Published: 1990

Total Pages: 340

ISBN-13:

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The first edition, published in 1982, became the standard source of electrophoresis protocols for nucleic acid researchers. This updated edition takes account of the advances made in refining established techniques and details many new techniques, including pulse field electrophoresis, gel retardation analysis, and DNA footprinting. Annotation copyrighted by Book News, Inc., Portland, OR

Nucleic Acid Electrophoresis

Nucleic Acid Electrophoresis PDF

Author: Dietmar Tietz

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 341

ISBN-13: 3642589243

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Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described here in detail. Leading experts present the required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers' addresses, helpful Internet resources, as well as specific applications, e.g. in legal medicine, microbiology and agriculture.

Gel Electrophoresis: Nucleic Acids

Gel Electrophoresis: Nucleic Acids PDF

Author: Dr Robin Martin

Publisher: Garland Science

Published: 2020-08-26

Total Pages: 190

ISBN-13: 1000101436

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Through its clear presentation of the basic concepts, Gel Electrophoresis: Nucleic Acids breaks new ground by describing the principles of the technique without resorting to complicated protocols and recipes.

Gel Electrophoresis

Gel Electrophoresis PDF

Author: Sameh Magdeldin

Publisher: BoD – Books on Demand

Published: 2012-04-04

Total Pages: 380

ISBN-13: 9535104586

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Most will agree that gel electrophoresis is one of the basic pillars of molecular biology. This coined terminology covers a myriad of gel-based separation approaches that rely mainly on fractionating biomolecules under electrophoretic current based mainly on the molecular weight. In this book, the authors try to present simplified fundamentals of gel-based separation together with exemplarily applications of this versatile technique. We try to keep the contents of the book crisp and comprehensive, and hope that it will receive overwhelming interest and deliver benefits and valuable information to the readers.

Gel Electrophoresis: Nucleic Acids

Gel Electrophoresis: Nucleic Acids PDF

Author: P. Jones

Publisher: Wiley-Blackwell

Published: 1995

Total Pages: 198

ISBN-13:

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Gel electrophoresis of nucleic acids is the one technique that spans the whole range of molecular biology techniques. The combination of its high resolution and versatility of its applications makes it the one method used by all molecular biologists. This book gives clear, step-by-step protocols for all the important techniques from simple analytical separations of nucleic acids to the latest PCR techniques. Hence it will be essential reading for all those working in the area of molecular biology. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.

DNA Electrophoresis

DNA Electrophoresis PDF

Author: Svetlana Makovets

Publisher: Humana

Published: 2016-08-23

Total Pages: 0

ISBN-13: 9781493959983

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In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.

Analysis of Nucleic Acids by Capillary Electrophoresis

Analysis of Nucleic Acids by Capillary Electrophoresis PDF

Author: Christoph Heller

Publisher: Springer Science & Business Media

Published: 2013-06-29

Total Pages: 318

ISBN-13: 3322910156

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Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.

Capillary Electrophoresis of Nucleic Acids

Capillary Electrophoresis of Nucleic Acids PDF

Author: Keith R. Mitchelson

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 481

ISBN-13: 1592590551

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The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

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