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Molecular Methods in Ecology

Author: Allan Baker

Publisher: John Wiley & Sons

Published: 2009-04-01

Total Pages: 349

ISBN-13: 1444311417

The incorporation of molecular methods in ecological research has added an exciting new dimension to conventional studies, and opened windows into previously intractable areas of research, at the interface between ecology and genetics. Using these new methods it has now become routine to use genetic markers to study ecological phenomena, from molecular sexing of individuals and parentage of offspring, through to population structure of species and phylogenetic relationships of taxa. These methods have stimulated an explosion of empirical and analytical developments in molecular ecology, which have in turn, increasingly attracted students and professional biologists eager to employ them in their studies. Molecular Methods in Ecology traces the development of molecular ecology by reviewing basic molecular biological techniques and earlier methods such as protein electrophoresis, DNA-DNA hybridisation, restriction analysis of DNA, and DNA fingerprinting. Later chapters review methods using newer classes of markers such as microsatellites, introns, MHC, SSRs and AFLP markers in plants and molecular sexing in animals. The strengths and limitations of methods are discussed and guidance is provided in selecting the most appropriate methods for particular problems in ecology. This book will provide both postgraduates and researchers with a guide to choosing and employing appropriate methodologies for successful research in the field of molecular ecology. Provides up-to-date summaries of the latest molecular approaches in this rapidly expanding field. Gives guidance on the appropriate choice of methods for particular problems in ecology, and their strengths and limitations. Provides brief laboratory protocols for each molecular method and summaries of software available for analysis of data in molecular ecology. Outlines examples of the latest research results from studies of both plants and animals, integrated within the framework of molecular ecology.

Molecular Microbial Ecology

Author: Mark Osborn

Publisher: Taylor & Francis

Published: 2004-06-02

Total Pages: 256

ISBN-13: 1135320411

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Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary method of culturing microorganisms on media allowed us to study only between 0.1 and 10% of the total microbial flora in any given environment. Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world’s leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.

Molecular Methods for Evolutionary Genetics

Author: Virginie Orgogozo

Publisher: Humana Press

Published: 2011-03-15

Total Pages: 503

ISBN-13: 9781617792274

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We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology.

Molecular Ecology and Evolution: Approaches and Applications

Author: B. Schierwater

Publisher: Birkhäuser

Published: 2013-06-29

Total Pages: 626

ISBN-13: 3034875274

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The past 25 years have witnessed a revolution in the way ecologists and evolutionary biologists approach their disciplines. Modern molecular techniques are now reshaping the spectrum of questions that can be addressed while studying the mechanisms and consequences of the ecology and evolution of living organisms. "Molecular Ecology and Evolution: Approaches and Applications" describes, from a molecular perspective, several methodological and technical approaches used in the fields of ecology, evolution, population biology, molecular systematics, conservation genetics, and development. Modern techniques are introduced, and older, more classic ones refined. The advantages, limitations, and potentials of each are discussed in detail, and thereby illustrate the widening range of cross-field research and applications which this modern technology is stimulating. This book will serve as an important textbook for graduate and advanced undergraduate students, and as a key reference work for researchers

Molecular Techniques in the Microbial Ecology of Fermented Foods

Author: Luca Cocolin

Publisher: Springer Science & Business Media

Published: 2007-12-15

Total Pages: 291

ISBN-13: 0387745203

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With the application of new analytical techniques, the field of food fermentation has grown in recent years. This book provides the latest information and relevant advances on the microbial ecology of fermented foods and the application of molecular methods. This book serves as a guide for students and researchers on the most advanced techniques to identify bacteria and helps in choosing the most appropriate tools to study fermented food from a microbiological point of view.

Current Protocols in Molecular Biology

Author:

Publisher:

Published:

Total Pages:

ISBN-13: 9780471503385

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Molecular Approaches To Ecology And Evolution

Author: R. deSalle

Publisher: Springer Science & Business Media

Published: 1998-09-29

Total Pages: 390

ISBN-13: 9783764357252

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"The last ten years have seen an explosion of activity in the application of molecular biological techniques to evolutionary and ecological studies. This volume attempts to summarize advances in the field and place into context the wide variety of methods available to ecologists and evolutionary biologists using molecular techniques. Both the molecular techniques and the variety of methods available for the analysis of such data are presented in the text. The book has three major sections - populations, species and higher taxa. Each of these sections contains chapters by leading scientists working at these levels, where clear and concise discussion of technology and implication of results are presented. The volume is intended for advanced students of ecology and evolution and would be a suitable textbook for advanced undergraduate and graduate student seminar courses." -- Publisher.

Molecular Techniques in Food Biology

Author: Aly Farag El Sheikha

Publisher: John Wiley & Sons

Published: 2018-01-02

Total Pages: 472

ISBN-13: 1119374618

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Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability explores all aspects of microbe-food interactions, especially as they pertain to food safety. Traditional morphological, physiological, and biochemical techniques for the detection, differentiation, and identification of microorganisms have severe limitations. As an alternative, many of those responsible for monitoring food safety are turning to molecular tools for identifying foodborne microorganisms. This book reviews the latest molecular techniques for detecting, identifying, and tracing microorganisms in food, addressing both good foodborne microbes, such as those used for fermentation and in probiotics, and harmful ones responsible for foodborne illness and food quality control problems. Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability brings together contributions by leading international authorities in food biology from academe, industry, and government. Chapters cover food microbiology, food mycology, biochemistry, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and food science and technology. Throughout, special emphasis is placed on novel molecular techniques relevant to food biology research and for monitoring and assessing food safety and quality. Brings together contributions from scientists at the leading edge of the revolution in molecular food biology Explores how molecular techniques can satisfy the dire need to deepen our understanding of how microbial communities develop in foods of all types and in all forms Covers all aspects of food safety and hygiene, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and more Fills a yawning gap in the world literature on food traceability using molecular techniques This book is an important working resource for professionals in agricultural, food science, biomedicine, and government involved in food regulation and safety. It is also an excellent reference for advanced students in agriculture, food science and food technology, biochemistry, microbiology, and biotechnology, as well as academic researchers in those fields.

Molecular Microbial Ecology Manual

Author: Antoon D. L. Akkermans

Publisher: Springer

Published: 2014-01-13

Total Pages: 266

ISBN-13: 9400902158

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For a long time microbial ecology has been developed as a distinct field within Ecology. In spite of the important role of microorganisms in the environment, this group of 'invisible' organisms remained unaccessable to other ecologists. Detection and identification of microorganisms remain largely dependent on isolation techniques and characterisation of pure cul tures. We now realise that only a minor fraction of the microbial com munity can be cultivated. As a result of the introduction of molecular methods, microbes can now be detected and identified at the DNA/RNA level in their natural environment. This has opened a new field in ecology: Molecular Microbial Ecology. In the present manual we aim to introduce the microbial ecologist to a selected number of current molecular techniques that are relevant in micro bial ecology. The first edition of the manual contains 33 chapters and an equal number of additional chapters will be added this year. Since the field of molecular ecology is in a continuous progress, we aim to update and extend the Manual regularly and will invite anyone to depo sit their new protocols in full detail in the next edition of this Manual. We hope this book finds its place where it was born: at the lab bench! Antoon D.L. Akkermans, Jan Dirk van Elsas and Frans J. de Bruijn March 1995 Molecular Microbial Ecology Manual 1.3.6: 1-8, 1996. © 1996 Kluwer Academic Publishers.

Molecular Ecology

Author: Joanna R. Freeland

Publisher: John Wiley & Sons

Published: 2006-03-30

Total Pages: 403

ISBN-13: 0470090634

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Molecular Ecology provides a comprehensive introduction to the many diverse aspects of this subject. The book unites theory with examples from a wide range of taxa in a logical and progressive manner, and its accessible writing style makes subjects such as population genetics and phylogenetics highly comprehensible to its readers. The first part of the book introduces the essential underpinnings of molecular ecology, starting with a review of genetics and a discussion of the molecular markers that are most frequently used in ecological research. This leads into an overview of population genetics in ecology. The second half of the book then moves on to specific applications of molecular ecology, covering phylogeography, behavioural ecology and conservation genetics. The final chapter looks at molecular ecology in a wider context by using a number of case studies that are relevant to various economic and social concerns, including wildlife forensics, agriculture, and overfishing * comprehensive overview of the different aspects of molecular ecology * attention to both theoretical and applied concerns * accessible writing style and logical structure * numerous up-to-date examples and references This will be an invaluable reference for those studying molecular ecology, population genetics, evolutionary biology, conservation genetics and behavioural ecology, as well as researchers working in these fields.