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Microbial and Plant Protoplasts
Plant Protoplasts
Author: Tessa Bengochea
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 97
ISBN-13: 9400940955
DOWNLOAD EBOOK →Isolated plant protoplasts are 'naked' cells that have had their cell wall removed either by mechanical action or by enzymic digestion. As a result of wall removal the only barrier that exists between the cell protoplasm and the external environment is the plasma membrane. The removal of the cell wall has drastic osmotic consequences for the isolated cell. Rather like animal cells, the isolated protoplasts must be maintained in an osmotically balanced (isotonic) nutrient medium or they will burst. As will be seen later, the isolation of plant protoplasts is not a particularly new event; as early as the 1890s scientists were isolating protoplasts mechanically and studying effects such as protoplast streaming. What has brought protoplast technology to the forefront of plant biology is the potential application of these structures in non-traditional plant improvement methods. Extreme care must be exercised by the reader, however, in be lieving some of the claims that have been put forward for the use of protoplasts in improvement programmes. In the 20 years since the first experiments in routine enzyme isolation of large numbers of protoplasts no real improvement has been obtained in a commercial crop plant. This does not mean that improvement is impossible; it simply indicates that it may be a longer term objective than some people would like to admit.
Fungal Protoplasts
Author: J. Peberdy
Publisher: CRC Press
Published: 1985-02-20
Total Pages: 378
ISBN-13: 9780824771126
DOWNLOAD EBOOK →Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.
Protoplasts 1983
Author: Ingo Potrykus
Publisher: Birkhäuser
Published: 1983
Total Pages: 280
ISBN-13:
DOWNLOAD EBOOK →Recent developments in the culture and regeneration of plant protoplasts; Protoplast culture and plant regeneration of cereals and other recalcitrant crops; Protoplasts and the isolation of plant mutants; Protoplasts and variation from culture; Systems for plant protoplast transformation; Recent developments in plant protoplast fusion and selection technology; Somatic hybridization by plant protoplast fusion; The segregation of organelles and cytoplasmic traits in higher plant somatic fusion hybrids; The first mitotic cycle of mesophyll protoplasts; Protoplasts for studies of the plasma membrane and associated cell organelles; The use of protoplasts in plant virus research; Applications of protoplast technology to agriculture; Organelle transfer, sorting out, recombination; Plant protoplasts as tools for physiological studies; Genetic transformation; Protoplasts as tools in pathology, virology and plant-microbe interactions; Current questions of gene transfer via protoplast fusion in microorganisms (Opening adress); Advances in protoplast fusion and transformation in Streptomyces; Chromosome interactions and expression in fused Bacillus protoplasts; Yeast strain improvement by protoplast fusion and transformation; Protoplasts of filamentous fungi in genetics and metabolite production; Interspecific somatic hybridisation in Aspergillus; Synthesis and assembly of wall polymers on regenerating yeast protoplasts; The theory and practical applications of liposome-protoplast interactions; Transport of nutrients in yeast protoplast; Protoplasts and spheroplasts of gram-negative bacteria - with special emphasis on proteus mirabilis; Characteristic properties and biological significance of stable protoplast type L-forms; Advances in microbial protoplasts (Bacillus licheniformis lactamase and the protoplast surface); Fungal protoplasts as genetic tools; Non-complementing diploids: prokaryotic microorganism protoplast fusion and chromosome inactivation.
Plant Protoplasts
Author: Fowke
Publisher: CRC Press
Published: 2018-01-18
Total Pages: 254
ISBN-13: 1351084224
DOWNLOAD EBOOK →The present book thus emphasizes the importance of plant protoplasts for fundamental research. This book is intended to be used b senior undergraduates, graduate students, and research scientists in plant biology.
Yeast, Mould and Plant Protoplasts
Author: Julio Rodríguez Villanueva
Publisher:
Published: 1973
Total Pages: 416
ISBN-13:
DOWNLOAD EBOOK →Yeast cell wall lytic ensymes; Structure and morphogenesis; Physiology and biochemistry of yeast protoplasts; Protoplasts of moulds; Protoplasts of plants.
Genetic Engineering of Plants and Microorganisms Important for Agriculture
Author: E. Magnien
Publisher: Springer Science & Business Media
Published: 1985-02-28
Total Pages: 214
ISBN-13: 9789024731312
DOWNLOAD EBOOK →A Seminar held in the Framework of the Biomolecular Engineering Programma of the Commission of the European Communities, at the Carlsberg Laboratory in Copenhagen, October 9-10, 1984
Progress in Plant Protoplast Research
Author: K.J. Puite
Publisher: Springer
Published: 1988-05-31
Total Pages: 412
ISBN-13: 9024736889
DOWNLOAD EBOOK →In the series of International Protoplast Symposia the Symposium of 1987 was held in Wageningen (The Netherlands). Earlier Symposia took place in Jena (DDR) 1963, Brno (CSSR) 1967, Salamanca (Spain) 1971, Nottingham (UK) 1975, Szeged (Hungary) 1979 and Basel (Switzerland) 1983. This 7th International Protoplast Symposium was organized by K.J. Puite (Secretary), J.J.M. Dons (Treasurer), H.J.Huizing and E.J.L. Hotke-Staal (Local Organizers), the first three persons being scientists, respectively, from the Research Institute Ital, the Institute for horticultural plant breeding IVT and the Foundation for agricultural plant breeding SVP at Wageningen. Scientific Advisers of the Symposium were A. J. Kool, M. Koornneef and F.A. Krens. The International Agricultural Centre lAC served as the Symposium location. The Organizing Committee decided that the scientific programme of the Symposium should be mainly focussed on protoplast technology of relevance to plant breeding. Therefore research on microbial protoplasts and on secondary metabolites was not included. About 250 scientists from 27 different countries were welcomed at the meeting. Speakers at Symposium Sessions and authors of Poster contributions were asked to hand over their manuscripts for the Symposium Proceedings already at the meeting, permit ting early publication of the Proceedings. These manuscripts give the state of the art of the protoplast research and illustrate the progress since the last Protoplast Symposium.
Plant-microbe Interactions
Author: Tsune Kosuge
Publisher:
Published: 1984
Total Pages: 464
ISBN-13:
DOWNLOAD EBOOK →Overview. Concepts and terminology in plant-microbe interactions. Factors in pathogenesis. Microbial enzyne regulation and its importance for pathogenicity. Genetics of host-parasite systems: a prospectus for molecular biology. Approaches and tools for research. Diagnostic approaches for the rapid and specific detection of plant viruses and viroids. Theory and practice of genetic engineering. Development of plant vectors. Mutant selection. Molecular biology of recognition. Concepts and experimental approaches in host-microbe recognition. Adsorption of bacteria to plant surfaces. Plant response to the environment. Genetics of rhizobium nodulation. Systemic responses to wounding. Genetic and molecular aspects of resitance induced by infections or chemicals. Plant tumorigenesis. Biological control. Epiphytic microbes as biological control agents. Hypovirulence. A model to explain the "Cross protection" phenomenson shown by plant viruses and viroids.
