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Metabolomics in Practice

Author: Michael Lämmerhofer

Publisher: John Wiley & Sons

Published: 2013-02-14

Total Pages: 423

ISBN-13: 3527655883

Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment. Based on more than a decade of practical experience in developing, optimizing and validating metabolomics approaches as a routine technology in the academic and industrial research laboratory, the lessons taught here are highly relevant for all systems-level approaches, whether in systems biology, biotechnology, toxicology or pharmaceutical sciences. From the Contents: * Sampling and Sample Preparation in Microbial Metabolomics * Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics * GC-MS, LC-MS, CE-MS and Ultrahigh Resolution MS (FTICR-MS) in Metabolomics * NMR-based metabolomics analysis * Potential of Microfluidics and Single Cell Analysis in Metabolomics * Data Processing in Metabolomics * Validation and Measurement Uncertainty in Metabolomic Studies * Metabolomics and its Role in the Study of Mammalian Systems and in Plant Sciences * Metabolomics in Biotechnology and Nutritional Metabolomics and more.

Metabolomics Data Processing and Data Analysis-Current Best Practices

Author: Justin Van Der Hooft

Publisher: Mdpi AG

Published: 2021-09-10

Total Pages: 276

ISBN-13: 9783036511948

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Metabolomics data analysis strategies are central to transforming raw metabolomics data files into meaningful biochemical interpretations that answer biological questions or generate novel hypotheses. This book contains a variety of papers from a Special Issue around the theme "Best Practices in Metabolomics Data Analysis". Reviews and strategies for the whole metabolomics pipeline are included, whereas key areas such as metabolite annotation and identification, compound and spectral databases and repositories, and statistical analysis are highlighted in various papers. Altogether, this book contains valuable information for researchers just starting in their metabolomics career as well as those that are more experienced and look for additional knowledge and best practice to complement key parts of their metabolomics workflows.

Metabolomics

Author: Jens Nielsen

Publisher: Springer Science & Business Media

Published: 2007-09-19

Total Pages: 292

ISBN-13: 3540747192

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Giving a fresh, substantial and in-depth overview of the topic, this book brings together the latest results in the field of metabolomics. It comprehensively presents the current state of the metabolomics field by underscoring experimental methods, analysis techniques, standardization practices, and advances in specific model systems. As a result, it helps to significantly broaden our perspective on the principles and strategies underpinning this emerging field.

Metabolomics

Author: Ron Wehrens

Publisher: CRC Press

Published: 2019-08-19

Total Pages: 313

ISBN-13: 1315353482

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Metabolomics is the scientific study of the chemical processes in a living system, environment and nutrition. It is a relatively new omics science, but the potential applications are wide, including medicine, personalized medicine and intervention studies, food and nutrition, plants, agriculture and environmental science. The topics presented and discussed in this book are based on the European Molecular Biology Organization (EMBO) practical courses in metabolomics bioinformatics taught to those working in the field, from masters to postgraduate students, PhDs, postdoctoral and early PIs. The book covers the basics and fundamentals of data acquisition and analytical technologies, but the primary focus is data handling and data analysis. The mentioning and usage of a particular data analysis tool has been avoided; rather, the focus is on the concepts and principles of data processing and analysis. The material has been class-tested and includes lots of examples, computing and exercises. Key Features: Provides an overview of qualitative /quantitative methods in metabolomics Offers an introduction to the key concepts of metabolomics, including experimental design and technology Covers data handling, processing, analysis, data standards and sharing Contains lots of examples to illustrate the topics Includes contributions from some of the leading researchers in the field of metabolomics with extensive teaching experiences

Metabolomics: From Fundamentals to Clinical Applications

Author: Alessandra Sussulini

Publisher: Springer

Published: 2017-01-28

Total Pages: 350

ISBN-13: 3319476564

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This book provides a comprehensive view of metabolomics, from the basic concepts, through sample preparation and analytical methodologies, to data interpretation and applications in medicine. It is the first volume to cover metabolomics clinical applications while also emphasizing analytical and statistical features. Moreover, future trends and perspectives in clinical metabolomics are also presented. For researches already experienced in metabolomics, the book will be useful as an updated definitive reference. For beginners in the field and graduate students, the book will provide detailed information about concepts and experimental aspects in metabolomics, as well as examples and perspectives of applications of this strategy to clinical questions.

Metabolomics

Author: Wolfram Weckwerth

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 290

ISBN-13: 1597452440

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Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Applied Environmental Metabolomics

Author: David J. Beale

Publisher: Academic Press

Published: 2022-06-09

Total Pages: 448

ISBN-13: 0128164611

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Applied Environmental Metabolomics: Community Insights and Guidance from the Field brings together contributions from global experts who have helped to define and develop the exciting and rapid advances that are taking place in the field of environmental metabolomics. This book is aimed at expert users, students, researchers, and academics in metabolomics and systems biology. It not only demonstrates the best practice in experimental design but also provides insight into state-of-the-art instrumentation and the depth of analysis one can expect to get by using various sampling, chromatographic, mass spectrometric, and nuclear magnetic resonance (NMR) techniques. Common experimental and technical pitfalls are also highlighted. This book provides a unique insight into the world of environmental metabolomics and will help the practicing scientist avoid repeating similar costly mistakes, steering them efficiently toward the generation of high-quality data and high-impact publications. Highlights overarching principles and considerations for researchers to leverage when planning, conducting, and evaluating environmental metabolomics research Applies key insights and lessons learned from leaders in the field Provides real-world case study applications of multiple environmental metabolomics techniques Integrates the Metabolomics Standards Initiative into case study examples Encompasses standard operating protocols for metabolomics to help new entrants to the field

Methodologies for Metabolomics

Author: Norbert W. Lutz

Publisher: Cambridge University Press

Published: 2013-01-21

Total Pages: 641

ISBN-13: 0521765900

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Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

Sample Preparation in Metabolomics

Author: Julia Kuligowski

Publisher: MDPI

Published: 2021-04-07

Total Pages: 206

ISBN-13: 3039438131

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Metabolomics is increasingly being used to explore the dynamic responses of living systems in biochemical research. The complexity of the metabolome is outstanding, requiring the use of complementary analytical platforms and methods for its quantitative or qualitative profiling. In alignment with the selected analytical approach and the study aim, sample collection and preparation are critical steps that must be carefully selected and optimized to generate high-quality metabolomic data. This book showcases some of the most recent developments in the field of sample preparation for metabolomics studies. Novel technologies presented include electromembrane extraction of polar metabolites from plasma samples and guidelines for the preparation of biospecimens for the analysis with high-resolution μ magic-angle spinning nuclear magnetic resonance (HR-μMAS NMR). In the following chapters, the spotlight is on sample preparation approaches that have been optimized for diverse bioanalytical applications, including the analysis of cell lines, bacteria, single spheroids, extracellular vesicles, human milk, plant natural products and forest trees.

Microbiome and Metabolome in Diagnosis, Therapy, and other Strategic Applications

Author: Joel Faintuch

Publisher: Academic Press

Published: 2019-01-03

Total Pages: 506

ISBN-13: 0128152508

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Microbiome and Metabolome in Diagnosis, Therapy, and Other Strategic Applications is the first book to simultaneously cover the microbiome and the metabolome in relevant clinical conditions. In a pioneering fashion, it addresses not only the classic intestinal environment, but also the oral, gastric, lung, skin and vaginal microbiome that is in line with the latest investigations. Nonbacterial microbiomes, such as fungi and viruses are not overlooked, and the plasma microbiome is also discussed. As plasma, brain, placenta, tumor cells, and other sterile fluids and tissues, are increasingly recognized to potentially host a microbiome, albeit a limited one, this is a timely resource. The book's editors were fortunate to have the input of renowned collaborators from nearly all continents. This is truly an international effort that brings the latest in the field to students and professionals alike. Provides comprehensive coverage on diagnosis, therapy, pharmacotherapy and disease prevention in context of the microbiome and metabolome Focuses on the proposed physiological or pathological conditions Presents an up-to-date, useful reference