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Lymph Node Fine-needle Cytology and Flow Cytometry in Clinical Practice

Author: Pio Zeppa

Publisher:

Published: 2016

Total Pages: 0

ISBN-13: 9783318059403

Lymph node (LN) fine-needle cytology (FNC) coupled with flow cytometry immunophenotyping provides important information for diagnosing non-Hodgkin lymphoma. This special topic issue offers comprehensive reviews that describe the state of the art of LN FNC-FC and all its possible applications. FNC and FC are two independent procedures that can conveniently meet and merge in specific conditions and for specific purposes. FNC samples are, for example, suitable for various molecular procedures. The issue includes a brief description of available tools and their potential application to LN FNC. FNC may be useful for diagnosing lymphoma and determining prognosis. As such, this publication is highly recommended to cytopathologists, pathologists, and hematologists.

Lymph Node Cytopathology

Author: Stefan E. Pambuccian

Publisher: Springer Science & Business Media

Published: 2010-10-17

Total Pages: 292

ISBN-13: 1441969640

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This volume in the Essentials in Cytopathology book series will focus on the cytopathology of lymph nodes. It will address the topic of fine needle aspiration of lymph nodes and fulfill the need for an easy-to-use and authoritative synopsis of lymph node cytopathology. The book with adopt an algorithmic diagnostic approach, starting from the cytomorphologic pattern of the lymph node aspirate. The focus will be on the appropriate and effective use of ancillary studies (immunohistochemistry, flow cytometry, fluorescence in situ hybridization and molecular techniques) and integration of their results into the final diagnosis. The book will present the cytopathologic features and differential diagnoses for the major cytologic patterns in lymph node fine needle aspiration. The entities typically falling within each of these patterns will be discussed with illustration of the spectrum of cytologic features, differential diagnoses and pitfalls. The book will cover the full spectrum of benign and malignant primary conditions of the lymph nodes, with emphasis on common disorders. Discussion of metastatic conditions will be restricted to those that are relevant to the differential diagnosis of primary lymphoid disorders. This book is designed as an easy to use resource that fits into the lab coat pocket, ideal for portability and quick reference. It is heavily illustrated and contains useful algorithms that guide the reader through the differential diagnosis of common and uncommon entities encountered in lymph node aspirates. The accompanying text follows an easy to use pattern-based outline. A useful resource for every pathologist, cytopathologist, fellow and trainee.

FNA Cytology in the Diagnosis of Lymphoma

Author: Lambert Skoog

Publisher: Karger Medical and Scientific Publishers

Published: 2009

Total Pages: 89

ISBN-13: 3805586264

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A comprehensive color manual Fine-needle aspiration (FNA) became important for the cytology of the enlarged lymph node in the 1950s and 1960s and was accepted in the diagnosis of various types of lymphadenitis and metastatic disease. The diagnosis of lymphoma by FNA cytology, however, remained controversial for many years, as FNA smears did not allow the evaluation of growth pattern. Only later with the introduction of immunocytochemistry on FNA material it became possible to conclusively diagnose the majority of lymphomas with an accuracy comparable to that of histopathology. Other ancillary techniques such as FISH and PCR can now also be applied successfully to FNA material. These facts together with the excellent clinical performance of FNA sampling should increase the spread of the technique. This comprehensive manual presents the cytomorphologic and immunocytochemical characteristics of both non-Hodgkin and Hodgkin lymphomas. It discusses the technical, methodological aspects of lymphoma diagnosis and describes the cytologic features of reactive lymphoid lesions and the major types of neoplastic lymphoid lesions, based on the most recent (2001) WHO lymphoma classification. Key cytologic and immunologic features are listed to facilitate a conclusive diagnosis of the different lesions. This publication will be of immense value to clinicians such as cytopathologists, pathologists, oncologists, and hematologists involved in the clinical work-up and management of patients with lymph node lesions.

Lymph Node FNC

Author: P. Zeppa

Publisher: Karger Medical and Scientific Publishers

Published: 2017-11-14

Total Pages: 116

ISBN-13: 3318061158

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This well-illustrated monograph presents a comprehensive description of Fine-Needle Cytology (FNC), including technical procedures and ancillary techniques, applied to the lymph node (LN). Possibilities and limitations are highlighted to produce accurate cytological diagnosis of LN and cytological criteria, and to be exploited for extra-nodal lymphoproliferative processes. The normal LN structure and corresponding cytological patterns are described. Cytological, phenotypical, and molecular features are reviewed along with diagnostic criteria for various medical conditions such as lymphadenitis and lymphadenopathies, Hodgkin and Non-Hodgkin lymphoma, pediatric lymphoadenopaties, immunodeficiency-associated lymphoproliferative disorders, extra-nodal lymphoproliferative processes, metastases, and other non-lymphomatous processes. LN-FNC requires knowledge of hemathopathology and imaging techniques, as well as dexterity with FNC performing, smearing, and material management. Rapid on-site evaluation (ROSE), the choice of ancillary techniques and molecular procedures case by case are required to produce accurate and comprehensive diagnosis. This book will help cytopathologists to conduct these tasks in various organs and clinical contexts. LN-FNC requires knowledge of hemathopathology and imaging techniques, as well as dexterity with FNC performing, smearing, and material management. Rapid on-site evaluation (ROSE), the choice of ancillary techniques and molecular procedures case by case are required to produce accurate and comprehensive diagnosis. This book will help cytopathologists to conduct these tasks in various organs and clinical contexts.

Frozen Section Library: Lymph Nodes

Author: Cherie H. Dunphy

Publisher: Springer Science & Business Media

Published: 2011-10-27

Total Pages: 132

ISBN-13: 1461412536

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The Frozen Section Library series provides concise, user-friendly, site specific handbooks that are well illustrated and highlight the pitfalls, artifacts and differential diagnosis issues that arise in the hurried frozen section scenario. Frozen Section Library: Lymph Nodes highlights the usefulness of techniques such as touch preparation cytology and fine needle aspiration (FNA) cytology, as well as FNA biopsy. This fascicle also suggests proper handling for subsequent ancillary studies, including flow cytometric, cytogenetic, and molecular studies. Special emphasis is given to the limitations of frozen section diagnosis in lymph node pathology. All chapters are written by experts in their fields and include the most up to date scientific information. This book serves as a very useful resource for physicians in the frozen section room and in intra-operative consultation situations dealing with, and interested in, this very complex field of diagnostic pathology. Frozen Section Library: Lymph Nodes is of great value to pathologists, residents, and fellows who diagnose pathologic processes involving lymph nodes. Cherie H. Dunphy, M.D. is Professor of Pathology and Laboratory Medicine, Executive Director of Hematopathology and Hematopathology Fellowship Department of Pathology and Laboratory Medicine University of North Carolina Chapel Hill, NC.

Differential Diagnosis in Cytopathology Book and Online Bundle

Author: Paolo Gattuso

Publisher: Cambridge University Press

Published: 2014-12-04

Total Pages: 695

ISBN-13: 1107040299

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This updated edition remains the essential text for pathologists seeking to make accurate diagnoses from the vast number of differentials.

Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration (EBUS-TBNA): A Practical Approach

Author: S.E. Monaco

Publisher: Karger Medical and Scientific Publishers

Published: 2014-05-22

Total Pages: 147

ISBN-13: 3318023965

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This high-yield reference book focuses on the clinical, technical, and pathological aspects of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA). Its reviews cover all aspects of EBUS-TBNA, including the clinical perspective, technical aspects of the procedure, and cytomorphology of common and uncommon entities, as well as highlights diagnostic challenges. Each chapter features a multitude of full-color high-resolution images and includes key references to the current literature in the field. Additionally, reference tables and informative figures highlight the salient points. The book is unique in that it is written by experienced thoracic surgeons, pulmonary medicine physicians, and cytopathologists who use EBUS-TBNA in a large medical center. This publication is of interest to individuals learning and practicing cytopathology, in addition to clinicians practicing pulmonary/thoracic medicine or surgery. In short, it provides important pearls of wisdom to create a comprehensive reference for all physicians involved with EBUS-TBNA.

Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies

Author: Anna Porwit

Publisher: Cambridge University Press

Published: 2018-01-25

Total Pages: 248

ISBN-13: 1108548830

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Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.

Veterinary Cytology

Author: Leslie C. Sharkey

Publisher: John Wiley & Sons

Published: 2020-08-04

Total Pages: 1024

ISBN-13: 1119125723

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This book offers a state-of-the-art, evidence-based reference to all aspects of veterinary cytology. Truly multidisciplinary in its approach, chapters are written by experts in fields ranging from clinical pathology to internal medicine, surgery, ophthalmology, and dermatology, drawing the various specialties together to create a comprehensive picture of cytology's role in diagnosis and treatment of animal disease. Firmly grounded in the primary literature, the book focuses on companion animals, with special chapters for species with fewer publications. Chapters are logically organized by body system, with additional chapters on tumors of particular import and diagnostic decision making. The first two sections of Veterinary Cytology focus on cytology techniques, quality control, and special laboratory techniques. Subsequent sections are organ/tissue-based and reflect what is known about the canine, feline, and equine species. This is followed by chapters on non-traditional species, including exotic companion mammals, rabbits, cattle, camelids, non-human primates, reptiles and birds, amphibians, fish, invertebrates, and sheep and goats. The last section highlights some unique features of the applications of cytology in industry settings. Provides a gold-standard reference to data-driven information about cytologic analysis in companion animal species Brings together authors from a wide range of specialties to present a thorough survey of cytology's use in veterinary medicine Offers broader species coverage and greater depth than any cytology reference currently available Veterinary Cytology is an essential resource for clinical and anatomic pathologists and any specialist in areas using cytology, including veterinary oncologists, criticalists, surgeons, ophthalmologists, dermatologists, and internists.

Fine Needle Aspiration Cytology

Author: Gabrijela Kocjan

Publisher: Springer Science & Business Media

Published: 2006-02-23

Total Pages: 243

ISBN-13: 3540314121

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Histopathologists all over the world have to report cytopathology during the course of their work and it is then that they find themselves facing diagnostic dilemmas. This practical, well-illustrated book, explicitly dedicated to this readership will serve their needs and meets their requirements in daily practice.