Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation

Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation PDF

Author: Stepan Podzimek

Publisher: John Wiley & Sons

Published: 2011-04-20

Total Pages: 325

ISBN-13: 111810272X

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A comprehensive, practical approach to three powerful methods of polymer analysis and characterization This book serves as a complete compendium of three important methods widely used for the characterization of synthetic and natural polymers—light scattering, size exclusion chromatography (SEC), and asymmetric flow field flow fractionation (A4F). Featuring numerous up-to-date examples of experimental results obtained by light scattering, SEC, and A4F measurements, Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation takes an all-in-one approach to deliver a complete and thorough explanation of the principles, theories, and instrumentation needed to characterize polymers from the viewpoint of their molar mass distribution, size, branching, and aggregation. This comprehensive resource: Is the only book gathering light scattering, size exclusion chromatography, and asymmetric flow field flow fractionation into a single text Systematically compares results of size exclusion chromatography with results of asymmetric flow field flow fractionation, and how these two methods complement each other Provides in-depth guidelines for reproducible and correct determination of molar mass and molecular size of polymers using SEC or A4F coupled with a multi-angle light scattering detector Offers a detailed overview of the methodology, detection, and characterization of polymer branching Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation should be of great interest to all those engaged in the polymer analysis and characterization in industrial and university research, as well as in manufacturing quality control laboratories. Both beginners and experienced can confidently rely on this volume to confirm their own understanding or to help interpret their results.

Field-Flow Fractionation in Biopolymer Analysis

Field-Flow Fractionation in Biopolymer Analysis PDF

Author: S. Kim R. Williams

Publisher: Springer Science & Business Media

Published: 2011-12-07

Total Pages: 306

ISBN-13: 3709101530

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This is a timely collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems. Importantly, the field-flow fractionation (FFF) technique has recently emerged as a prominent complement to size exclusion chromatography for protein pharmaceuticals. Fractionation with FFF is gentle and preserves protein structural integrity better than existing alternatives. In the present text, different chapters are written by experts in their respective field of application, who offer comparisons between the FFF techniques and other methods for characterizing their special focus material. Practical guide-lines for successful implementation, such as choice of operating conditions, are offered in conjunction with each application. In addition to new instrumentation and approaches that address important current topics, readers are provided with an overall sense of prior (but timeless) major developments that may be overlooked in literature searches.

Field-Flow Fractionation Handbook

Field-Flow Fractionation Handbook PDF

Author: Martin E. Schimpf

Publisher: John Wiley & Sons

Published: 2000-08-02

Total Pages: 618

ISBN-13: 9780471184300

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Field flow fractionation (FFF) is an emerging separation technique, which has been proven successful in the analysis of pharmaceuticals, biotechnology products, polymers, soils, and foods, among others. In this book, Martin Schimpf joins forces with Karin Caldwell and J. Calvin Giddings, two of the primary developers of this technique, to bring you the first comprehensive, one-stop reference on the technique.

Field-Flow Fractionation

Field-Flow Fractionation PDF

Author: Josef Janca

Publisher: CRC Press

Published: 2022-11-08

Total Pages: 349

ISBN-13: 1000715566

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Field-flow fractionation has become a very effective method for the separation and analytical characterization of substances of macromolecular and particulate character-making this method probably the most important discovery in the analytical separation field since World War II. The first, complete, up-to-date reference on this notable separation method, Field-Flow Fractionation includes comprehensive explanations of general and theoretical principles . . . presents detailed descriptions of experimental techniques and instrumentation ... and discusses advantages over competitive processes. In addition, this resource demonstrates various applications for characterizing synthetic polymers ... analyzing biopolymers and particles in environmental samples ... and determining sizes of biological cells, viruses, and subcellular particles. Substantiating main conclusions of theories, experiments, and applications with graphs and drawings, and including numerous tables and photographs, this one-source reference is invaluable reading for analytical, industrial, physical, and polymer chemists; chrom­ atographers; biochemists; biotechnologists; biophysicists; and all others interested in the study and characterization of macromolecules or particles of various origins.

Nanoemulsions in Food Technology

Nanoemulsions in Food Technology PDF

Author: Javed Ahmad

Publisher: CRC Press

Published: 2021-10-17

Total Pages: 326

ISBN-13: 1000454479

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As of late, greater efforts are being made in the use of nanoemulsion techniques to encapsulate, protect, and deliver functional compounds for food applications, given their advantages over conventional emulsification techniques. In addition, delivery systems of nano-scale dimensions use low-energy emulsification methods and exclude the need of any solvent, heat, or sophisticated instruments in their production. Divided into three sections, Nanoemulsions in Food Technology: Development, Characterization, and Applications will provide in-depth information and comprehensive discussion over technologies, physical and nanostructural characterization, as well as applicability of the nanoemulsion technique in food sciences. It describes the techniques involved in nanoemulsion characterization, mainly dealing with interfacial and nanostructural characterization of nanoemulsions, different physical characterization techniques, as well as various imaging and separation techniques involved in its characterization. Key Features Provides a detailed discussion about the technology of nanoemulsion Explains how nanoemulsion technique is helpful in using essential oils of different biological sources Presents methods of preparation and recent advancements in manufacturing along with stability perspectives of this technique. Discusses recent advancements in manufacturing and reviews the stability perspectives of nanoemulsion techniques This book contains in-depth information on a technology overview, physical and nanostructural characterization, as well as applicability of the nanoemulsion technique in food sciences. It is a concise body of information that is beneficial to researchers, industries, and students alike. The contributing authors are drawn from a rich blend of experts in various areas of scientific field exploring nanoemulsion techniques for wider applications. Also available in the Food Analysis and Properties Series: Sequencing Technologies in Microbial Food Safety and Quality, edited by Devarajan Thangardurai, Leo M.L. Nollet, Saher Islam, and Jeyabalan Sangeetha (ISBN: 9780367351182) Chiral Organic Pollutants: Monitoring and Characterization in Food and the Environment, edited by Edmond Sanganyado, Basil K. Munjanja, and Leo M.L. Nollet (ISBN: 9780367429232) Analysis of Nanoplastics and Microplastics in Food, edited by Leo. M.L. Nollet and Khwaja Salahuddin Siddiqi (ISBN: 9781138600188)

Thermal Field-Flow Fractionation of Polymers

Thermal Field-Flow Fractionation of Polymers PDF

Author: Guilaume Greyling

Publisher: Springer

Published: 2019-02-21

Total Pages: 116

ISBN-13: 3030106500

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This book introduces the reader to thermal field-flow fractionation and discusses its advantages over the fundamental problems associated with traditional column-based analytical techniques commonly used to characterize polymers and macromolecules. The authors discuss the theoretical background, equipment, experimental procedures as well as the recent advances and applications of thermal field-flow fractionation. Complete with several practical examples and troubleshooting guidelines, the book is written for beginners and experienced separation scientists alike and will enable its readers to optimize their experimental conditions for their specific separation needs and problems.

New and Emerging Proteomic Techniques

New and Emerging Proteomic Techniques PDF

Author: Dobrin Nedelkov

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 237

ISBN-13: 159745026X

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Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Modern Size-Exclusion Liquid Chromatography

Modern Size-Exclusion Liquid Chromatography PDF

Author: Andre Striegel

Publisher: John Wiley & Sons

Published: 2009-07-31

Total Pages: 512

ISBN-13: 0470442832

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The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.

Light Scattering from Polymer Solutions and Nanoparticle Dispersions

Light Scattering from Polymer Solutions and Nanoparticle Dispersions PDF

Author: Wolfgang Schärtl

Publisher: Springer Science & Business Media

Published: 2007-08-13

Total Pages: 200

ISBN-13: 3540719512

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Light scattering is a very powerful method for characterizing the structure of polymers and nanoparticles in solution. As part of the Springer Laboratory series, this book provides a simple-to-read and illustrative textbook probing the seemingly very complicated topic of light scattering from polymers and nanoparticles in dilute solution, and goes further to cover some of the latest technical developments in experimental light scattering.