Light Microscopy in Biology

Light Microscopy in Biology PDF

Author: Alan J. Lacey

Publisher: OUP Oxford

Published: 1999-06-03

Total Pages: 475

ISBN-13: 0191565725

DOWNLOAD EBOOK →

Since the first edition of Light Microscopy in Biology: A Practical approach was published, techniques in modern light microscopy have improved considerably. This fully updated edition includes revised topics from the first edition as well as coverage of techniques and technologies that have been developed since it was published. As before, the book starts with an explanation of the basic techniques, and goes on to describe current methods in: chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building and video microscopy. Totally new topics covered include: confocal microscopy, calcium and pH imaging, microinjection techniques and nanovid microscopy. There are also whole chapters now devoted to reflection contrast microscopy and histomorphometry. This new edition will be of great interest to postgraduate and postdoctoral researchers in biomedicine and cell biology - both those experienced with light microscopic techniques and newcomers to the field.

Introduction to Light Microscopy

Introduction to Light Microscopy PDF

Author: Dee Lawlor

Publisher: Springer

Published: 2019-05-31

Total Pages: 164

ISBN-13: 3030053938

DOWNLOAD EBOOK →

This book offers a beginner’s guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyone with a general interest in these valuable tools.

Light Microscopy

Light Microscopy PDF

Author: David J. Rawlins

Publisher: Taylor & Francis

Published: 1992

Total Pages: 160

ISBN-13:

DOWNLOAD EBOOK →

This is a straightforward and comprehensive guide to the practical use of the light microscope for the examination of biological specimens. Written in an informal style, it aims to be a readable explanation of all the types of light microscopy currently in use in the modern biological laboratory. It is designed to be used at the bench, next to the microscope. Theoretical explanations are kept to the minimum necessary to support the practical information.

Basic Methods in Microscopy

Basic Methods in Microscopy PDF

Author: David L. Spector

Publisher: CSHL Press

Published: 2006

Total Pages: 384

ISBN-13: 0879697512

DOWNLOAD EBOOK →

This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.

Biomedical Light Microscopy

Biomedical Light Microscopy PDF

Author: J. James

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 200

ISBN-13: 9401137781

DOWNLOAD EBOOK →

New interest in light microscopy of the last few years has not been backed up by adequate general literature. This book intends to fill the gap between specialized texts on detailed topics and general introductory booklets, mostly dealing with the use of the conventional light microscope only. In this short textbook both new developments in microscopy and basic facts of image formation will be treated, including often neglected topics such as axial resolving power, lens construction, photomicrography and correct use of phase-en interference contrast systems. Theoretical background will be dealt with as far as necessary for a well-considered application of these techniques enabling a deliberate choice for the approach of a certain problem. Over 150 illustrations (photomicrographs and diagrams) complete the information on microscopy of the nineties in the biomedical field, intended for scientists, doctors, technicians and research students. Many drawings have been contributed by the illustrator R. Kreuger; the photographic work has been executed by J. Peeterse. Secretarial assistance in preparing the manuscript was given by Ms T. M. S. Pierik. Dr M. J. Pearson has corrected the English of the final text.

Digital Microscopy

Digital Microscopy PDF

Author: Greenfield Sluder

Publisher: Elsevier

Published: 2007-04-26

Total Pages: 626

ISBN-13: 0080544347

DOWNLOAD EBOOK →

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. Expands coverage to include discussion of confocal microscopy not found in the previous edition Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy PDF

Author: James Pawley

Publisher: Springer Science & Business Media

Published: 2013-04-17

Total Pages: 639

ISBN-13: 1475753489

DOWNLOAD EBOOK →

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Light Microscopy

Light Microscopy PDF

Author: Hélio Chiarini-Garcia

Publisher: Humana Press

Published: 2010-12-15

Total Pages: 0

ISBN-13: 9781607619499

DOWNLOAD EBOOK →

Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.

Lanterna Rally - Theme by Grace Themes