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Light microscopic techniques in biology and medicine

Author: J. James

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 345

ISBN-13: 9401014140

Up to about twenty-five years ago, virtually the entire field of microscopy could be overseen and even practized by any active research worker. The rapid evolution which microscopy in its broadest sense has since undergone and which has contributed greatly to our insight in many fields of biological science and medicine has, however, lead to a progressive specialisation. Both experienced investigators in clinical and biological laboratories and post graduate students, confronted with a limited number of microscopic tech niques in their daily research work, have increasing difficulty in keeping (or obtaining) a general idea of the many time-honoured and new possibilities which microscopy has to offer. This book has been written with the aim of presenting general informa tion on light microscopic techniques, at a level somewhere in between booklets like those provided by microscope manufacturers (which are often too much focussed on the production program of a particular make) and very advanced treatises with a thorough mathematical treatment of all phenomena concerned. The physically oriented texts moreover often do not sufficiently take into account the practical situation in a medical or biolog ical laboratory; on the other hand, the value of really understanding what one is doing in using a microscopic technique is often underestimated. At tempt has been made, therefore, to present sufficient background informa tion necessary for a rational application of the different microscopical tech niques in their mutual relationship.

Techniques In Microscopy For Biomedical Applications

Author: Terje Dokland

Publisher: World Scientific Publishing Company

Published: 2006-09-28

Total Pages: 422

ISBN-13: 9813101954

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The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.

Basic Methods in Microscopy

Author: David L. Spector

Publisher: CSHL Press

Published: 2006

Total Pages: 384

ISBN-13: 0879697512

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This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.

Biomedical Light Microscopy

Author: J. James

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 200

ISBN-13: 9401137781

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New interest in light microscopy of the last few years has not been backed up by adequate general literature. This book intends to fill the gap between specialized texts on detailed topics and general introductory booklets, mostly dealing with the use of the conventional light microscope only. In this short textbook both new developments in microscopy and basic facts of image formation will be treated, including often neglected topics such as axial resolving power, lens construction, photomicrography and correct use of phase-en interference contrast systems. Theoretical background will be dealt with as far as necessary for a well-considered application of these techniques enabling a deliberate choice for the approach of a certain problem. Over 150 illustrations (photomicrographs and diagrams) complete the information on microscopy of the nineties in the biomedical field, intended for scientists, doctors, technicians and research students. Many drawings have been contributed by the illustrator R. Kreuger; the photographic work has been executed by J. Peeterse. Secretarial assistance in preparing the manuscript was given by Ms T. M. S. Pierik. Dr M. J. Pearson has corrected the English of the final text.

Advanced Techniques in Biological Electron Microscopy

Author: J.K. Koehler

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 319

ISBN-13: 3642654924

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The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.

Molecular Biology of The Cell

Author: Bruce Alberts

Publisher:

Published: 2002

Total Pages: 0

ISBN-13: 9780815332183

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Biological Electron Microscopy

Author: Michael J. Dykstra

Publisher: Springer Science & Business Media

Published: 2011-06-28

Total Pages: 537

ISBN-13: 1441992448

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Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.

Bioimaging

Author: Douglas E. Chandler

Publisher: Jones & Bartlett Publishers

Published: 2009

Total Pages: 468

ISBN-13: 0763738743

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The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.

Light Microscopy

Author: Hélio Chiarini-Garcia

Publisher: Humana Press

Published: 2010-12-15

Total Pages: 0

ISBN-13: 9781607619499

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Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.

Microscopy

Author: Terence Allen

Publisher: Oxford University Press, USA

Published: 2015

Total Pages: 153

ISBN-13: 0198701268

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Using light, electrons, or X-rays, microscopes today form a vital tool not only in biology but in many other disciplines, including materials science and nanotechnology. In this Very Short Introduction Terence Allen describes the scientific principles behind the main forms of microscopy, and the exciting new developments in the field. Beginning with a brief history of microscopy, Allen surveys the diverse and powerful forms of miscroscopes available today, illustrating how microscopy impinges on almost every aspect of our daily lives.