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Author: Lubiniecki
Publisher: Routledge
Published: 2018-05-02
Total Pages: 656
ISBN-13: 135143571X
DOWNLOAD EBOOK →An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator
Author: Bjorn K. Lydersen
Publisher:
Published: 1987
Total Pages: 252
ISBN-13: 9780029488812
DOWNLOAD EBOOK →Author: Lubiniecki
Publisher: Routledge
Published: 2018-05-02
Total Pages: 412
ISBN-13: 1351435701
DOWNLOAD EBOOK →An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator
Author: Joseph Feder
Publisher: Elsevier
Published: 2012-12-02
Total Pages: 170
ISBN-13: 0323160786
DOWNLOAD EBOOK →Large-Scale Mammalian Cell Culture is composed of papers presented as part of a symposium sponsored by the American Chemical Society Division of Microbial and Biochemical Technology at the 188th American Chemical Society National Meeting, held at Philadelphia, Pa., on Aug. 27, 1984. A rapid development of large-scale mammalian cell culture technology for the production of biologically important molecules becomes apparent. This book looks into this technology, its potential for commercial application, and the regulatory concerns posed by its use for the production of human therapeutics.
Author: Bjorn K. Lydersen
Publisher:
Published: 1987
Total Pages: 252
ISBN-13: 9781569900543
DOWNLOAD EBOOK →The Book This book provides the most detailed and comprehensive survey available of the different methods for production of biotechnology products from large scale mammalian and plant cell culture. Methods ranging from fermentation and encapsulation to hollow fibers and fluidized beds are described by the leaders in the field with a section on regulatory considerations. Each production method is described in detail in terms of principles, equipment and results so that informed comparisons and evaluations can be made. Contents Antibody Production with Airlift Fermentors ò Monoclonal Antibody Production in Stirred Reactors ò Microcarrier Cell Culture ò Cellular Microencapsulation for Large-Scale Production of Monoclonal Antibodies ò Entrapment of Cultured Cells in Agarose Beads ò An Automated Hollow Fiber System for the Large Scale Manufacture of Mammalian Cell Secreted Product ò Continuous Cell Culture with Fluidized Sponge Beads ò Perfusion Cell Culture System Based on Ceramic Matrices ò Large Scale Plant Cell Culture ò Safety Considerations for Cell Culture-Derived Biologicals
Author: Michael Butler
Publisher: Garland Science
Published: 2004-08-02
Total Pages: 299
ISBN-13: 1135325375
DOWNLOAD EBOOK →Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.
Author: Mohamed Al-Rubeai
Publisher: Springer Science & Business Media
Published: 2009-08-11
Total Pages: 259
ISBN-13: 9048122457
DOWNLOAD EBOOK →Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.
Author: Sadettin Ozturk
Publisher: CRC Press
Published: 2005-08-30
Total Pages: 788
ISBN-13: 0849351065
DOWNLOAD EBOOK →Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology devel
Author: R. E. Spier
Publisher: Butterworth-Heinemann
Published: 2013-09-24
Total Pages: 853
ISBN-13: 1483165159
DOWNLOAD EBOOK →Production of Biologicals from Animal Cells in Culture reviews the state of the art in animal cell biotechnology, with emphasis on the sequence of events that occur when generating a biological from animal cells in culture. Methods that enable adjustment of nutrient feed streams into perfusion bioreactors so as to increase productivity are described. A number of issues are also addressed, such as the usefulness of the fingerprint method for cell characterization. Comprised of 135 chapters, this book begins with an overview of the problems and benefits of animal cell culture, followed by a discussion on the isolation of immortal murine macrophage cell lines. The reader is systematically introduced to the use of DNA fingerprinting to characterize cell banks; immortalization of cells with oncogenes; lipid metabolism of animal cells in culture; and energetics of glutaminolysis. Subsequent chapters explore serum-free and protein-free media; the physiology of animal cells; gene expression in animal cell systems; and animal cell bioreactors. The monitoring and assay of animal cell parameters are also considered, along with downstream processing and regulatory issues. This monograph will be of interest to students, practitioners, and investigators in the fields of microbiology and biotechnology.
Author: Gyun Min Lee
Publisher: John Wiley & Sons
Published: 2020-01-13
Total Pages: 436
ISBN-13: 3527343342
DOWNLOAD EBOOK →Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.
