Handbook of Detection of Enzymes on Electrophoretic Gels

Handbook of Detection of Enzymes on Electrophoretic Gels PDF

Author: Gennady P. Manchenko

Publisher: CRC Press

Published: 2002-12-26

Total Pages: 1137

ISBN-13: 100065415X

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Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish

Handbook of Detection of Enzymes on Electrophoretic Gels

Handbook of Detection of Enzymes on Electrophoretic Gels PDF

Author: Gennady P. Manchenko

Publisher: CRC Press

Published: 2002-12-26

Total Pages: 568

ISBN-13: 1420040537

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Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish

Electrophoresis of Enzymes

Electrophoresis of Enzymes PDF

Author: Gunter M. Rothe

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 314

ISBN-13: 3642790690

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The electrophoresis of enzymes and isoenzymes is a well established technique in biochemical, clinical, environmental, microbiological, botanical and forensic laboratories and classical electrophoresis is presently undergoing a remarkable revival. This book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. The author summarizes them in clearly readable tables, in many carefully worked out electrophoresis and more than 140 staining protocols. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every enzyme laboratory. It will supply the practitioner with profound information on state-of-the-art enzyme electrophoresis.

Pulsed Field Gel Electrophoresis

Pulsed Field Gel Electrophoresis PDF

Author: Bruce Birren

Publisher: Academic Press

Published: 2012-12-02

Total Pages: 272

ISBN-13: 0323137628

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Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. Reviews all types of pulsed field gel electrophoresis Describes all commercially available systems and summarizes advantages and limitations of each Includes step-by-step protocols for sample preparation and analysis Illustrated with photographs that depict How to run gels: What the results should look like What they look like when they go wrong Covers applications to a wide range of organisms Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism

Molecular Genetic Analysis of Populations

Molecular Genetic Analysis of Populations PDF

Author: A. R. Hoelzel

Publisher: Oxford University Press

Published: 1998-03-19

Total Pages: 468

ISBN-13: 0191565601

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Methods enabling the direct study of genetic variation in natural populations have improved considerably. The new edition explores these updated techniques in DNA analysis and provides a revised and refined laboratory guide to investigating variation in DNA molecules.

Gel Electrophoresis of Proteins

Gel Electrophoresis of Proteins PDF

Author: Michael J Dunn

Publisher: Elsevier

Published: 2014-05-12

Total Pages: 422

ISBN-13: 1483193543

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Gel Electrophoresis of Proteins focuses on the techniques, methodologies, reactions, and approaches involved in gel electrophoresis of proteins. The selection first covers steady-state gel electrophoresis systems and one-dimensional PAA-gel electrophoretic techniques to separate functional and denatured proteins. Discussions focus on affinity electrophoresis, structure and physico-chemical properties of polyacrylamide gels, moving boundary electrophoresis, isotachophoresis, fundamental steady-state electrophoresis systems, and fundamental properties of steady-state electrophoresis systems. The text then reviews conventional isoelectric focusing and immobilized pH gradients and high resolution two-dimensional polyacrylamide-gel electrophoresis. Topics include production of narrow pH gradients, extended pH gradients, polymerization kinetics, IEF in agarose matrices, titration curves, two-dimensional electrophoresis under non-denaturing conditions, and IEF in polyacrylamide matrices. The book tackles quantifying patterns from two-dimensional PAGE, protein staining and detection methods, and immunoelectrophoretic methods. Concerns include post-electrophoretic organic protein stains, silver and enzyme stains, detection of radioactive proteins, and programming languages. The selection is highly recommended for researchers wanting to conduct studies on gel electrophoresis of proteins.

A Laboratory Manual on Rhipicephalus microplus

A Laboratory Manual on Rhipicephalus microplus PDF

Author: Raquel Cossio-Bayugar

Publisher: Cambridge Scholars Publishing

Published: 2023-05-03

Total Pages: 364

ISBN-13: 1527504298

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Discussing all aspects connected with the scientific analysis of Rhipicephalus microplus, this book covers tick classification and identification, as well as methods of extracting natural products effective against ticks. It also describes tick cell culture procedures, tick acaricide-resistance diagnostics, and the identification of tick parasites and microorganisms from the host and the ticks’ fluids, as well as the diagnosis of Babesia and Anaplasma in R. microplus.

Electrophoresis in Practice

Electrophoresis in Practice PDF

Author: Reiner Westermeier

Publisher: John Wiley & Sons

Published: 2016-03-16

Total Pages: 458

ISBN-13: 3527695168

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This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis. The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.

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