Gene Cloning and Manipulation

Gene Cloning and Manipulation PDF

Author: C. J. Howe

Publisher: Cambridge University Press

Published: 1995-03-31

Total Pages: 228

ISBN-13: 9780521407007

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"Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduate, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations."--BOOK JACKET.

Gene Cloning and Manipulation

Gene Cloning and Manipulation PDF

Author: Christopher Howe

Publisher: Cambridge University Press

Published: 2007-07-12

Total Pages: 250

ISBN-13: 1139463586

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Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Gene Cloning

Gene Cloning PDF

Author: David M. Glover

Publisher: Springer

Published: 2013-12-19

Total Pages: 231

ISBN-13: 1489932461

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This book was originallyconceived in the form ofa second edition ofa volume published in 1980 in Chapman and Hall's 'OutllneStudies in Biology' series and entitled Genetic Engineering - Cloning DNA. It very rapidly became apparent that with the impact ofrecombinant DNA techniques being feIt in so many areas ofblology, it was going to be difficultifnotimpossible to keepthe bookwithin the space confines of these little monographs. The stays were therefore loosened and the book expanded comfortably to its present size. I hope that this extra space has allowed me to clarify sections ofthe text that were 'heavy going' in the earlierversion. Theextraspace has certainlyallowed me to cover topics that were not mentioned at all in the earlier book. These are primarily to be found in Chapters 7 and 8, which cover the rapid advances that have been recently made in the use ofplantand animal cells as hosts for recombinant DNAmolecules. The develop ment ofother vectors has certainly not stood still over the past four years. This has necessitated a thorough revision ofChapters 3 and 4, which deal with bacteriophage and bacterial plasmid vectors. Numerous techniques for in vitromutagenesis have now been tried and tested allowing me to givecomprehensive coverage ofthisarea in Chapter 2 along with the biochemical techniques used to construct recombinant DNA molecules. Readers with some background knowledge of the approaches to gene cloning will be able to go straight toapart ofthe book in whichthey are specificallyinterested.

Textbook on Cloning, Expression and Purification of Recombinant Proteins

Textbook on Cloning, Expression and Purification of Recombinant Proteins PDF

Author: Kakoli Bose

Publisher: Springer Nature

Published: 2022-01-25

Total Pages: 315

ISBN-13: 9811649871

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This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.

Gene Cloning and Manipulation

Gene Cloning and Manipulation PDF

Author: Professor of Economics School of Oriental and African Studies Christopher Howe

Publisher:

Published: 2014-05-14

Total Pages: 278

ISBN-13: 9780511296536

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Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

The ABCs of Gene Cloning

The ABCs of Gene Cloning PDF

Author: Dominic Wong

Publisher: Springer Science & Business Media

Published: 2007-12-31

Total Pages: 227

ISBN-13: 0387286799

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Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning.

Gene Cloning

Gene Cloning PDF

Author: Terence A. Brown

Publisher: Nelson Thornes

Published: 1998

Total Pages: 0

ISBN-13: 9780748740703

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Gene Cloning provides a basic introduction for students and researchers who have no previous experience of experiments with DNA, and assumes very little prior knowledge on the part of the reader. A three part structure addresses the basic principles of gene cloning, the application of cloning in gene analysis, and the role of gene cloning in research and biotechnology. The book is written in clear, jargon-free language, and is extensively illustrated with two-color line drawings.

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