Methods in Cell Biology Vol. 29

Methods in Cell Biology Vol. 29 PDF

Author: Leslie Wilson

Publisher: Academic Press

Published: 1988-12

Total Pages: 0

ISBN-13: 9780125641296

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Annotation On preparation, delivery, and detection of fluorescent probes. Discusses factors which determine the destination of probes and methods for delivering them to specific sites in living cells. Includes issues related to sample physiology (microscopic cell culture), optics (basic fluorescence microscopy), and signal detection (electric photometry and imaging, immunoelectron microscopic detection of fluorophores). Annotation copyrighted by Book News, Inc., Portland, OR.

Fluorescence microscopy of living cells in culture

Fluorescence microscopy of living cells in culture PDF

Author:

Publisher:

Published: 1991-01

Total Pages: 333

ISBN-13: 9780126847543

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Parts A and B, companion volumes on fluorescence microscopy, are sourcebooks of methods for living cells. Part A comprehensively covers methodology: fluorescent analogs, procedures for fluorescent labeling of various cellular structures, and fluorescence microscopy. Part B describes instrumentation and quantitation analysis for imaging and spectroscopy. Key Features * Convenient bench-top format Comprehensive methods inluded for: * Tracing proteins, peptides, hormones, and toxins * Labeling cell surfaces, mitochondria, endoplasmic reticulum, and endocytic compartments * Preparation of fluorescent probes and their incorporation into cells * Culturing cells under the microscope * Basic fluorescence microscopy and imaging techniques

Fluorescence Methods for Investigation of Living Cells and Microorganisms

Fluorescence Methods for Investigation of Living Cells and Microorganisms PDF

Author: Natalia Grigoryeva

Publisher: BoD – Books on Demand

Published: 2020-09

Total Pages: 464

ISBN-13: 1839680393

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Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

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