Fluorescence Methods for Investigation of Living Cells and Microorganisms

Fluorescence Methods for Investigation of Living Cells and Microorganisms PDF

Author: Natalia Grigoryeva

Publisher: BoD – Books on Demand

Published: 2020-09

Total Pages: 464

ISBN-13: 1839680393

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Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

Methods in Cell Biology Vol. 29

Methods in Cell Biology Vol. 29 PDF

Author: Leslie Wilson

Publisher: Academic Press

Published: 1988-12

Total Pages: 0

ISBN-13: 9780125641296

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Annotation On preparation, delivery, and detection of fluorescent probes. Discusses factors which determine the destination of probes and methods for delivering them to specific sites in living cells. Includes issues related to sample physiology (microscopic cell culture), optics (basic fluorescence microscopy), and signal detection (electric photometry and imaging, immunoelectron microscopic detection of fluorophores). Annotation copyrighted by Book News, Inc., Portland, OR.

Fluorescence Microscopy in Life Sciences

Fluorescence Microscopy in Life Sciences PDF

Author: Juan Carlos Stockert

Publisher: Bentham Science Publishers

Published: 2017-12-15

Total Pages: 751

ISBN-13: 1681085186

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Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.

Studying Cyanobacteria by Means of Fluorescence Methods

Studying Cyanobacteria by Means of Fluorescence Methods PDF

Author: Natalia Yu Grigoryeva

Publisher:

Published: 2020

Total Pages: 0

ISBN-13:

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Self-fluorescence is a powerful tool for investigation of living photosynthetic microorganisms. Since the physiological state of such microorganisms is closely related to the operation and activity of photosynthetic system; thus, any variations in spectroscopic properties of their self-fluorescence indicate the changes in their physiological state. Cyanobacteria (or blue green alga) are one of the most widespread photosynthetic organisms in nature, and the ecological aspect in their investigation is quite valuable. On the other hand, thousands of strains belonging to different cyanobacterial species are cultivated in biolaboratories all over the world for different biotechnological applications such as biofuel cells, food production, pharmaceuticals, and fertilizers. Thus, the novel noninvasive methods of their investigation are quite important for on-line monitoring of cyanobacterial cultures. In this chapter, several fluorescence techniques are presented for investigation of living cyanobacterial cells and cultures.

Fluorescence Microscopy for Disease Diagnosis and Environmental Monitoring

Fluorescence Microscopy for Disease Diagnosis and Environmental Monitoring PDF

Author: Warren R. Sanborn

Publisher:

Published: 2005

Total Pages: 327

ISBN-13: 9789290213956

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Laboratories should be more aware of the advantages of using fluorescence microscopy. This manual provides information on the principles of fluorescence microscopy and practical advice on the preparation of samples for many simple applications for diagnosing disease and monitoring environmental contamination using a fluorescence microscope. The publication puts emphasis on procedures for direct, rapid identification of microorganisms causing a disease. The practical steps of indirect immunofluorescence microscopy for the diagnosis of noncommunicable diseases are also considered.

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