Enzyme Catalysis and Regulation

Enzyme Catalysis and Regulation PDF

Author: Gorden Hammes

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 276

ISBN-13: 032315817X

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Enzyme Catalysis and Regulation is an introduction to enzyme catalysis and regulation and covers topics ranging from protein structure and dynamics to steady-state enzyme kinetics, multienzyme complexes, and membrane-bound enzymes. Case studies of selected enzyme mechanisms are also presented. This book consists of 11 chapters and begins with a brief overview of enzyme structure, followed by a discussion on methods of probing enzyme structure such as X-ray crystallography and optical spectroscopy. Kinetic methods are then described, with emphasis on the general principles of steady-state and transient kinetics. The chemical principles involved in enzyme catalysis are also discussed, and case studies of a few well-documented enzymes are presented. The regulation of enzyme activity is analyzed from a nongenetic viewpoint, with particular reference to binding isotherms and models for allosterism. Two particular enzymes, aspartate transcarbamoylase and phosphofructokinase, are used as examples of well-studied regulatory enzymes. The last two chapters focus on multienzyme complexes and membrane-bound enzymes. This monograph is intended for graduate students, advanced undergraduates, and research workers in molecular biology and biochemistry.

ENZYMES: Catalysis, Kinetics and Mechanisms

ENZYMES: Catalysis, Kinetics and Mechanisms PDF

Author: N.S. Punekar

Publisher: Springer

Published: 2018-11-11

Total Pages: 562

ISBN-13: 9811307857

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This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.

Catalysis in Chemistry and Enzymology

Catalysis in Chemistry and Enzymology PDF

Author: William P. Jencks

Publisher: Courier Corporation

Published: 1987-01-01

Total Pages: 866

ISBN-13: 9780486654607

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Exceptionally clear coverage of mechanisms for catalysis, forces in aqueous solution, carbonyl- and acyl-group reactions, practical kinetics, more.

Enzyme Catalysis and Control

Enzyme Catalysis and Control PDF

Author: Marlene Deluca

Publisher: Elsevier

Published: 2014-06-28

Total Pages: 536

ISBN-13: 148321723X

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Current Topics in Cellular Regulation: Volume 24, Enzyme Catalysis and Control is a compendium of papers that discusses phosphoryl transfer reactions, the role of water on the free energy of hydrolysis of pyrophosphate, and the hormonal actions of vitamin D. Other papers describe the regulation lipid metabolism by a lipid-carrying protein, the GABA, and taurine enzymes in mammalian brain. One paper examines the role of vitamin D in the metabolism of cells, as well as in the whole animal. Upon absorption in the body, the vitamin undergoes various metabolic transformations before interacting with specific receptors, and then inducting the genome in the target tissues to generate biological and hormonal responses. Another paper notes the possibility of a genetic defect in cancer cells that results in the abnormal accumulation of sterol carrier protein (SCP) and cholesterol in vivo; and also in the inability to maintain levels of SCP or cholesterol in vitro. One paper shows that tartrate, or other organic acids, secreted into the medium by the penicillia keeps the pH in an optimal range for acid protease degradation of proteins and glycoproteins. This mechanism helps the fungus to survive in a nutrient environment (which is unbalanced with respect to an optimum C/N ratio and at a pH unfavorable to many bacteria and other life forms). Another paper proposes a model for the modulation of ATP synthetase activities and medium exchange reactions by energy input, substrate concentration, or others that affect the microenvironment of the enzyme under certain conditions. The compendium will prove beneficial to molecular biologists, general biologists, microbiologists, and biochemists.

Mechanisms of Catalysis

Mechanisms of Catalysis PDF

Author:

Publisher: Academic Press

Published: 1991-01-28

Total Pages: 459

ISBN-13: 9780080865966

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The remarkable expansion of information leading to a deeper understanding of enzymes on the molecular level necessitated the development of this volume which not only introduces new topics to The Enzymes series but presents new information on some covered in Volume I and II of this edition.

Enzyme Dynamics and Regulation

Enzyme Dynamics and Regulation PDF

Author: P. Boon Chock

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 441

ISBN-13: 1461237440

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Recent developments in concepts and techniques have brought enzyme research to a changing yet exciting stage. Enzymes have served as indispensable tools in the phenomenal rise of molecular biology, and the resultant biotechnology thrusts enzymes to new heights and territories. This volume, the proceedings of a recent symposium on the Dynamics of Soluble and Immobilized Enzyme Systems, provides a current overview of the field to help scientists utilize long-established and newly acquired information.

Enzyme Kinetics: Catalysis and Control

Enzyme Kinetics: Catalysis and Control PDF

Author: Daniel L. Purich

Publisher: Elsevier

Published: 2010-06-16

Total Pages: 920

ISBN-13: 0123809258

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Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

The Enzyme Catalysis Process

The Enzyme Catalysis Process PDF

Author: A. Cooper

Publisher: Springer Science & Business Media

Published: 2013-12-11

Total Pages: 479

ISBN-13: 1475716079

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This volume represents the proceedings of a NATO Advanced Studies Instituteheld near Barga (Italy), July 11-23, 1988, involving over 90 participants from more than twelve countries of Europe, North America and elsewhere. It was not our intention at this meeting to present a complete up-to-the-minute review of current research in enzyme catalysis but t·ather, in accord wi th the intended spiri t of NATO ASis, to gi ve an opportunity for advanced students and researchers in a wide variety of disciplines to meet tagether and study the problern from different points of view. Hence the lectures cover topics rauging from the purely theoretical aspects of chemical reaction kinetics in condensed matter through practical experimental approaches to enzyme structure, dynamics and mechanism, including the new experimental opportunities arising from genetic engineering techniques. Our approachwas unashamedly physical, both because the more biochemical aspects of enzymology are amply covered elsewhere and because progress in our understanding and application of the molecular basis of enzymic processes must ultimately come from advances in physical knowledge. We tried to cover as wide a spectrum as possible, and succeeded in gathering an expert and enthusiastic team of speakers, but there . are some inevitable omissions. In particular, and with hindsight, our discussions might have been enriched by more detailed coverage of general aspects of chemical catalysis - but readers requiring this background should find adequate references herein.

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