Entomopathogenic Nematodes and Their Symbiotic Bacteria

Entomopathogenic Nematodes and Their Symbiotic Bacteria PDF

Author: S. Sivaramakrishnan

Publisher: Springer

Published: 2022-05-07

Total Pages: 176

ISBN-13: 9781071614471

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This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

Entomopathogenic Nematodes and Their Symbiotic Bacteria

Entomopathogenic Nematodes and Their Symbiotic Bacteria PDF

Author: S. Sivaramakrishnan

Publisher: Springer

Published: 2021-06-08

Total Pages: 176

ISBN-13: 9781071614440

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This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

Entomopathogenic Nematodes and Their Symbiotic Bacteria

Entomopathogenic Nematodes and Their Symbiotic Bacteria PDF

Author: S. Sivaramakrishnan

Publisher:

Published: 2021

Total Pages: 176

ISBN-13: 9781071614457

DOWNLOAD EBOOK →

This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.

Entomopathogenic Nematodes: Systematics, Phylogeny and Bacterial Symbionts

Entomopathogenic Nematodes: Systematics, Phylogeny and Bacterial Symbionts PDF

Author: Khuong Nguyen

Publisher: BRILL

Published: 2007-09-30

Total Pages: 832

ISBN-13: 9047422392

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The volume deals with morphology, taxonomy and systematics of entomopathogenic nematodes (EPN) in the families Steinernematidae and Heterorhabditidae and bacteria associated with these nematodes. In the first part, history, taxonomic status, family and genus definitions of EPN are mentioned. In the second part, all useful aspects of morphology and methodology of EPN are mentioned with illustrations and SEM photographs and instructions on how to make the measurements, and how to use them in taxonomic work. A polychotomous key with SEM photographs and illustrations is provided. In the third part, full descriptions of all species are presented with latest information about each species. In the fourth part, molecular and phylogenetic methods for working with EPN are presented, including the latest information and instructions on how to use molecular data in taxonomic work. In the last part, bacteria associated with this group of nematodes are discussed in the context of the latest information about methodology, biology and taxon.

Entomopathogenic Nematodes

Entomopathogenic Nematodes PDF

Author: Khuong B. Nguyen

Publisher: BRILL

Published: 2007-01-01

Total Pages: 832

ISBN-13: 9004152938

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The volume deals with morphology, taxonomy and systematics of entomopathogenic nematodes (EPN) in the families Steinernematidae and Heterorhabditidae and bacteria associated with these nematodes. In the first part, history, taxonomic status, family and genus definitions of EPN are mentioned. In the second part, all useful aspects of morphology and methodology of EPN are mentioned with illustrations and SEM photographs and instructions on how to make the measurements, and how to use them in taxonomic work. A polychotomous key with SEM photographs and illustrations is provided. In the third part, full descriptions of all species are presented with latest information about each species. In the fourth part, molecular and phylogenetic methods for working with EPN are presented, including the latest information and instructions on how to use molecular data in taxonomic work. In the last part, bacteria associated with this group of nematodes are discussed in the context of the latest information about methodology, biology and taxon.

An Assessment of the Effects of Insect Host Condition on Entomopathogenic Nematodes and Their Symbiotic Bacteria

An Assessment of the Effects of Insect Host Condition on Entomopathogenic Nematodes and Their Symbiotic Bacteria PDF

Author: Victoria Alicia Miranda

Publisher:

Published: 2010

Total Pages: 134

ISBN-13:

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This study investigated a system comprised of entomopathogenic nematodes (EPN) (Steinernematidae and Heterorhabditidae), their symbiotic bacteria (Xenorhabdus, Photorhabdus) and an insect host, M. sexta. The focus of this investigation was to examine the effects insect host condition on the nematode-bacterium mutualistic partnership as a whole. The EPN used in the study had varying host-searching strategies: an ambusher, Steinernema carpocapsae, and a cruiser, Heterorhabditis sonorensis. Insect host age and diet were evaluated as factors that affect insect host condition. Wandering 5th instar M. sexta were much less susceptible to EPN infection compared to non-wandering 5th instar larvae, regardless of EPN species. Insect host diet had a significant impact on H. sonorensis susceptibility to EPN infection, and a non-significant trend was observed for S. carpocapsae. EPN and bacterial symbiont fitness were unaffected by insect host condition. This is the first record of the effect of insect host condition on both EPN and their symbiotic bacteria.

Entomopathogenic Nematodes in Biological Control

Entomopathogenic Nematodes in Biological Control PDF

Author: Randy Gaugler

Publisher: CRC Press

Published: 2018-01-18

Total Pages: 381

ISBN-13: 1351088645

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The editors of this book, who are world renown for their creativity with entomopathogenic nematodes, have assembled the foremost authorities from four continents to contribute on basic and applied concepts. The authors have taken advantage of this opportunity to express their views to a wide scientific audience. They have combined their international experience so that the latest developments in this fascinating and rapidly expanding field are present in comprehensive manner with diverse topics ranging from biological control theory to organismal and molecular biology.

Nematode Pathogenesis of Insects and Other Pests

Nematode Pathogenesis of Insects and Other Pests PDF

Author: Raquel Campos-Herrera

Publisher: Springer

Published: 2015-08-11

Total Pages: 532

ISBN-13: 3319182668

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Achieving a sustainable agriculture requires integrating advances in multiples disciplines, covering both fundamental and applied research in a common objective: enhancing crop health for better productions. This first volume of the Series “Sustainability in plant and crop protection” presents a comprehensive and multi-disciplinary compendium about the recent achievements in the use of entomopathogenic nematodes (EPNs) as biological control in a global scale. The volume is organized in a first section discussing the last discoveries on the biology and ecology of the EPN, a second section covering the advances on the EPN productions and release, and a third section with multiples case-studies in which the concepts and ideas on the two previous sections are integrated and discussed. An essential tool for researchers and professionals working to advance in the sustainable use of our resources.

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