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Author: Rajan Katoch
Publisher: Springer Science & Business Media
Published: 2011-07-19
Total Pages: 449
ISBN-13: 1441997857
DOWNLOAD EBOOK →Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.
Author: Veena Kumari
Publisher:
Published: 2013
Total Pages: 280
ISBN-13: 9789350840863
DOWNLOAD EBOOK →Author: Friedrich Lottspeich
Publisher: John Wiley & Sons
Published: 2018-05-29
Total Pages: 1151
ISBN-13: 3527339191
DOWNLOAD EBOOK →Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology
Author: Keith Wilson
Publisher: Cambridge University Press
Published: 2010-03-04
Total Pages: 769
ISBN-13: 0521516358
DOWNLOAD EBOOK →Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Author: Tai Te Wu
Publisher: Springer Science & Business Media
Published: 2007-05-08
Total Pages: 266
ISBN-13: 0306468921
DOWNLOAD EBOOK →Analytical Molecular Biology illustrates the importance of simple analytical methods applied to some basic molecular biology problems, with an emphasis on the importance of biological problems, rather than the complexity of mathematics. First, the book examines crucial experimental data for a specific problem. Mathematical models will then be constructed with explicit inclusion of biological facts. From such models, predictions can be deduced and then suggest further experimental studies. A few important molecular biology problems will be discussed in the order of the complexity of the mathematical models. Based on such illustrations, the readers can then develop their own analytical methods to study their own problems. This book is for anyone who knows they need to learn how to apply mathematical models to biology, but doesn't necessarily want to, from practicing researchers looking to acquire more analytical tools to advanced students seeking a clear, explanatory text.
Author: Helen Parkes
Publisher: Royal Society of Chemistry
Published: 2007-10-31
Total Pages: 209
ISBN-13: 1847559298
DOWNLOAD EBOOK →In spite of the wide variety and complexity of biological materials, nucleic acids are ubiquitous. DNA is becoming the bioanalyte of choice due to the vast amount of information embedded in its sequence, its robust chemical nature and the range of highly sensitive analytical techniques that have been developed. The results of such analyses can have an important impact on our society both commercially and in terms of the quality of life. Absolute confidence in the data generated is therefore of the utmost importance. This book, produced by LGC as part of the VAM (Valid Analytical Measurement) Programme, introduces the issues of validation and quality to the bioanalytical community, specifically addressing DNA-based analyses. It aims to raise awareness of the factors that can influence the validity of DNA analysis and the production of quality data. Emphasis is placed on VAM principles, as well as additional challenges that are associated with the analysis of real samples, for example, complex food matrices or forensic samples that have been subjected to environmental insult. Information is collated from a variety of sources including literature, discussions and LGC research, and offers constructive advice where possible.
Author: Thomas Rutherford
Publisher:
Published: 2019-08-23
Total Pages:
ISBN-13: 9781645320296
DOWNLOAD EBOOK →Analytical techniques such as spectroscopy, chromatography, etc. are particularly important in analytical biochemistry as well as in analytical chemistry generally. The principles of each technique are explained and the scope and applications are discussed. There are chapters on enzymes, antibodies and radio-isotopes, substances which it may be necessary to detect and measure but which also can be very useful in a variety of analytical methods. The present title "Principles of Analytical Biochemistry" is intended for those who wish to understand living organisms, especially man. Biochemistry is essential for this purpose, but it would be almost impossible for a student to survey on his own the massive body of existing knowledge, constantly augmented by a remarkable torrent of brilliant discoveries. Biochemistry is the study of the chemistry of living things. This includes organic molecules and their chemical reactions. Most people consider biochemistry to be synonymous with molecular biology. Biochemistry today has made spectacular progress in unraveling the mysteries of animate nature. This progress has allowed us to gain deeper insight into the principles of vital activity and has to a very significant extent stimulated the development of applied disciplines, especially medicine. The aim of this book is to present a core of biochemical knowledge that is desirable for undergraduate and postgraduate students and also those involved in the field of medical, microbiology, biotechnology and pharmaceutical.
Author: Mahin Basha
Publisher:
Published: 2020
Total Pages: 126
ISBN-13: 9781071601341
DOWNLOAD EBOOK →This book provides a comprehensive overview of the major biochemical analytical techniques, with detailed descriptions of the instrumentation and applications. The contributions, which each focus on a specific technique, are based on a thorough review and analysis of the current literature as well as the authors' experiences in the lab. Divided into nine parts, the book provides insights into basic separation techniques like sedimentation, filtration and centrifugation, as well as analytical techniques such as spectrophotometry, chromatography, electrophoresis, immuno-techniques, radioactivity and microscopy. .
Author: Markus R. Wenk
Publisher: Springer
Published: 2018-07-11
Total Pages: 0
ISBN-13: 9789400774667
DOWNLOAD EBOOK →The Encyclopedia of Lipidomics will present a complete overview of the field from fundamentals to new discoveries and from concepts, theories and experimental techniques to clinical and industrial applications. The book will develop with the active involvement of a strong editorial board comprised of leaders from the field. The Encyclopedia of Lipidomics intends to be a comprehensive reference resource serving to bridge the gap between clinical and basic science investigators and provide authoritative and digested information to students, scientists as well as non specialists. The book will have an edge over protocol type of works and databases in terms of having wider coverage through rigorous essays on terms, concepts, experimental and analytical techniques, applications and new challenges. Since lipidomics has strong linkages with several of the other biomedical and life sciences disciplines, the proposed encyclopedia will have a wide audience including graduate students, researchers and different levels of scientists in biomedicine, cellular and molecular biology, bioengineering, physiological and biochemistry, and pharmacology across both academia and industry.
Author: Robert Allen Meyers
Publisher: John Wiley & Sons
Published: 1995-06-29
Total Pages: 1090
ISBN-13: 9780471186342
DOWNLOAD EBOOK →This is one volume 'library' of information on molecular biology, molecular medicine, and the theory and techniques for understanding, modifying, manipulating, expressing, and synthesizing biological molecules, conformations, and aggregates. The purpose is to assist the expanding number of scientists entering molecular biology research and biotechnology applications from diverse backgrounds, including biology and medicine, as well as physics, chemistry, mathematics, and engineering.
