Electrochemistry of Nucleic Acids and Proteins

Electrochemistry of Nucleic Acids and Proteins PDF

Author: E. Palecek

Publisher: Elsevier

Published: 2005-12-19

Total Pages: 809

ISBN-13: 0080457452

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DNA (sometimes referred to as the molecule of life), is the most interesting and most important of all molecules. Electrochemistry of Nucleic Acids and Proteins: Towards Electrochemical Sensors for Genomics and Proteomics is devoted to the electrochemistry of DNA and RNA and to the development of sensors for detecting DNA damage and DNA hybridization. Volume 1, in the brand new series Perspectives in Bioanalysis, looks at the electroanalytical chemistry of nucleic acids and proteins, development of electrochemical sensors and their application in biomedicine and in the new fields of genomics and proteomics. The authors have expertly formatted the information for a wide variety of readers, including new developments that will inspire students and young scientists to create new tools for science and medicine in the 21st century. * Covers highly sophisticated methods of electrochemical analysis of nucleic acids and proteins * Summarises the present state of electrochemical analysis of nucleic acids and proteins * Includes future trends in the electrochemical analysis in genomics and proteomics

Electrochemistry of Nucleic Acids and Proteins

Electrochemistry of Nucleic Acids and Proteins PDF

Author: E. Palecek

Publisher: Elsevier

Published: 2005

Total Pages: 809

ISBN-13: 044452150X

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"This book is concerned with the electron transfer between electrodes on one hand and DNA, RNA and proteins on the other hand and with the use of electrochemistry and electrochemical sensors in DNA and protein analyses. Electrochemical bioassays involve newly emerging fields of genomic, proteomics, biomedicine and biotechnology. DNA and protein chips with electrochemical detection represent new tools of science, medicine and other areas of practical life in this century."--BOOK JACKET.

Bioelectrochemistry of Biomacromolecules

Bioelectrochemistry of Biomacromolecules PDF

Author: Giorgio Lenaz

Publisher: Birkhäuser

Published: 2012-12-06

Total Pages: 397

ISBN-13: 3034891792

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Bioelectrochemistry: Principles and Practice provides a comprehensive compilation of all the physicochemical aspects of the different biochemical and physiological processes. Macromolecules, essentially nucleic acids, proteins and complex carbohydrates, are the building blocks of cell structure and function. This fifth volume in the "Bioelectrochemistry" series deals essentially with water-soluble biomacromolecules, since the properties of membrane-bound proteins are considered in other volumes of this series. The first chapter provides an extensive review of the structure, chemical reactivity and electromagnetic properties of nucleic acids. The following five chapters concentrate on proteins, their structure, folding and function, the electrochemistry of redox proteins and voltammetric methods. Special attention is devoted to the field of thiol/disulfide exchange as well as to one particular class of proteins, the iron-sulfur proteins. The last chapter considers the chemistry and properties of glycosaminoglycans, the complex charged polysaccharides of the cell surface and extracellular matrix. This series is intended as a set of source books for graduate and postgraduate students as well as research workers at all levels in bioelectrochemistry.

Nucleic-Acid Electrochemistry

Nucleic-Acid Electrochemistry PDF

Author: Miroslav Fojta

Publisher: Springer

Published: 2012-07-28

Total Pages: 350

ISBN-13: 9783540699316

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This first monograph dedicated to the electrochemistry of nucleic acids offers complete coverage of the field. Modern electrochemical methods, in connection with different electrode types, are able to detect DNA, RNA or their synthetics analogues at attomole or even lower levels. In recent years, significant progress was achieved in the development of electrochemical techniques and sensors for DNA hybridization, DNA damage, interactions of nucleic acids with drugs, genotoxic species and other small molecules, as well as DNA-protein interactions. These sensors are expected to become important tools in biomedicine and other areas of practical life of the 21st century. Here, the authors describe the necessary experimental setup for electrochemical studies of nucleic acids, the properties and behavior of nucleic acids at electrode surfaces as well as the analytical applications of nucleic acid electrochemistry. All this information is presented with numerous worked examples and color figures.

Electrochemical DNA Biosensors

Electrochemical DNA Biosensors PDF

Author: Mehmet Sengun Ozsoz

Publisher: CRC Press

Published: 2012-04-23

Total Pages: 568

ISBN-13: 9814241776

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This book focuses on the basic electrochemical applications of DNA in various areas, from basic principles to the most recent discoveries. The book comprises theoretical and experimental analysis of various properties of nucleic acids, research methods, and some promising applications. The topics discussed in the book include electrochemical detection of DNA hybridization based on latex/gold nanoparticle and nanotubes; nanomaterial-based electrochemical DNA detection; electrochemical detection of microorganism-based DNA biosensors; gold nanoparticle-based electrochemical DNA biosensors; electrochemical detection of the aptamer-target interaction; nanoparticle-induced catalysis for DNA biosensing; basic terms regarding electrochemical DNA (nucleic acids) biosensors; screen-printed electrodes for electrochemical DNA detection; application of field-effect transistors to label free electrical DNA biosensor arrays; and electrochemical detection of nucleic acids using branched DNA amplifiers.

Methods of Protein and Nucleic Acid Research

Methods of Protein and Nucleic Acid Research PDF

Author: L.A. Osterman

Publisher: Springer

Published: 2012-04-13

Total Pages: 0

ISBN-13: 9783642874871

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Electrophoresis is the leading method among those used in the investigation of pro teins and nucleic acids. A paper on the study of these biopolymers without recourse to electrophoresis at each fractionation or characterization step is very unlikely to be encountered in the current scientific literature. This method enables separation of macromolecules according to characteristic features such as size (or molecular weight), shape, secondary structure and electric charge and these parameters can in fluence electrophoretic properties either separately or jointly. The physical background of this method is as follows. Macromolecules in a buf fer solution may become charged; the sign and magnitude of the total electrical charge depending on the pH of the medium. When such a solution is put into an insulated channel, e.g. in a glass tube, and a voltage applied at the ends of the chan nel, an electric field will be formed and direct current will pass through solution. The field strength can be defined as the potential difference at the ends of the chan nel (or its section) with respect to its length (v/cm). When exposed to an electrical field macromolecules will migrate towards the cathode or the anode according to their net charge and frictional force will limit the migration velocity.

Spherical Nucleic Acids

Spherical Nucleic Acids PDF

Author: Chad A. Mirkin

Publisher: CRC Press

Published: 2020-09-01

Total Pages: 1806

ISBN-13: 0429578067

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Spherical nucleic acids (SNAs) comprise a nanoparticle core and a densely packed and highly oriented nucleic acid shell, typically DNA or RNA. They have novel architecture-dependent properties that distinguish them from all other forms of nucleic acids and make them useful in materials synthesis, catalysis, diagnostics, therapeutics, and optics/plasmonics. This book covers over two decades of Dr. Mirkin’s research on SNAs and their anisotropic analogues, including synthesis and fundamental properties, and applications in colloidal crystallization, adaptive matter, and nanomedicine, spanning extra- and intracellular diagnostics, gene regulation, and immunomodulation. It is a reprint volume that compiles 101 key papers from high-impact journals in this research area published by the Mirkin Group at Northwestern University, Illinois, USA, within the International Institute for Nanotechnology, and collaborators. Volume 1 provides an overview and a historical framework of engineering matter from DNA-modified constructs and discusses the enabling features of nucleic acid–functionalized nanomaterials. Volume 2 covers design rules for colloidal crystallization, building blocks for crystal engineering, and DNA and RNA as programmable bonds. Volume 3 discusses colloidal crystallization processes and routes to hierarchical assembly, dynamic nanoparticle superlattices, surface-based and template-confined colloidal crystallization, optics and plasmonics with nanoparticle superlattices, and postsynthetic modification and catalysis with nanoparticle superlattices. Volume 4 covers diagnostic modalities, and intracellular therapeutic and diagnostic schemes based upon nucleic acid–functionalized nanomaterials.

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