-PDF Download- Dna Microarrays And Related Genomics Techniques EBOOK

DNA Microarrays and Related Genomics Techniques

Author: David B. Allison

Publisher: CRC Press

Published: 2005-11-14

Total Pages: 391

ISBN-13: 1420028790

Considered highly exotic tools as recently as the late 1990s, microarrays are now ubiquitous in biological research. Traditional statistical approaches to design and analysis were not developed to handle the high-dimensional, small sample problems posed by microarrays. In just a few short years the number of statistical papers providing approaches

DNA Microarrays

Author: Mark Schena

Publisher: Scion Publishing Ltd

Published: 2007-10-01

Total Pages: 289

ISBN-13: 1907904395

DOWNLOAD EBOOK →

DNA Microarrays: Methods Express covers the very latest in DNA microarray technology, with a clear focus on how these techniques can be used in the lab to gain the very best results. The authors are from some of the leading laboratories in the field and write with real authority on the latest methodology. Every chapter provides detailed step-by-step protocols with valuable hints and tips for success, as well as giving typical experimental results and selected literature citations. This book is a 'must have' manual for researchers in all fields of biology, medicine and agriculture.

Microarray Technology Through Applications

Author: Francesco Falciani

Publisher: Garland Science

Published: 2007-06-30

Total Pages: 276

ISBN-13: 0203967275

DOWNLOAD EBOOK →

Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology. The first chapter is a concise introduction to the technology and provides the theoretical background required to understand the subsequent sections. The following chapters are a series of case studies representative of the most general and important applications of microarray technology, including CGH, analysis of gene expression, SNP arrays and protein arrays. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. There are detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section. There is also information on printing glass DNA microarray slides and data interpretation. Colour figures and data sets are provided on the website at http://www.garlandscience.com/9780415378536

DNA Microarrays and Gene Expression

Author: Pierre Baldi

Publisher: Cambridge University Press

Published: 2011-04-28

Total Pages: 238

ISBN-13: 9781139437608

DOWNLOAD EBOOK →

Massive data acquisition technologies, such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine. Using the mRNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell. Such snapshots can be used to study fundamental biological phenomena such as development or evolution, to determine the function of new genes, to infer the role individual genes or groups of genes may play in diseases, and to monitor the effect of drugs and other compounds on gene expression. Originally published in 2002, this inter-disciplinary introduction to DNA arrays will be of value to anyone with an a interest in this powerful technology.

DNA Microarrays, Part A: Array Platforms and Wet-Bench Protocols

Author:

Publisher: Elsevier

Published: 2011-08-19

Total Pages: 506

ISBN-13: 0080464653

DOWNLOAD EBOOK →

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results. Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations. Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician. Provides an overview of platforms Includes experimental design and wet bench protocols Presents statistical and data analysis methods, array databases, data visualization and meta-analysis

Microarray Technology in Practice

Author: Steve Russell

Publisher: Academic Press

Published: 2008-11-21

Total Pages: 460

ISBN-13: 0080919766

DOWNLOAD EBOOK →

Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments Up-to-date description of normalization methods and current methods for sample amplification and labeling Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays

DNA Arrays

Author: Jang B. Rampal

Publisher: Springer Science & Business Media

Published: 2008-02-02

Total Pages: 268

ISBN-13: 1592592341

DOWNLOAD EBOOK →

Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .

DNA Arrays

Author: Elena V. Grigorenko

Publisher: CRC Press

Published: 2001-12-21

Total Pages: 183

ISBN-13: 1420038850

DOWNLOAD EBOOK →

A quantum leap in technology took place a few years ago with the introduction of cDNA arrays that have been developed in response to the need for simultaneous analysis of the patterns of expression of thousands of genes. DNA Arrays: Technologies and Experimental Strategies offers a view of different aspects of this rapidly developing technology, in

Computational and Statistical Approaches to Genomics

Author: Wei Zhang

Publisher: Springer Science & Business Media

Published: 2007-05-08

Total Pages: 345

ISBN-13: 0306478250

DOWNLOAD EBOOK →

Computational and Statistical Genomics aims to help researchers deal with current genomic challenges. Topics covered include: overviews of the role of supercomputers in genomics research, the existing challenges and directions in image processing for microarray technology, and web-based tools for microarray data analysis; approaches to the global modeling and analysis of gene regulatory networks and transcriptional control, using methods, theories, and tools from signal processing, machine learning, information theory, and control theory; state-of-the-art tools in Boolean function theory, time-frequency analysis, pattern recognition, and unsupervised learning, applied to cancer classification, identification of biologically active sites, and visualization of gene expression data; crucial issues associated with statistical analysis of microarray data, statistics and stochastic analysis of gene expression levels in a single cell, statistically sound design of microarray studies and experiments; and biological and medical implications of genomics research.

Functional Genomics

Author: Chris Town

Publisher: Springer Science & Business Media

Published: 2002-04-30

Total Pages: 222

ISBN-13: 9781402004568

DOWNLOAD EBOOK →

This volume provides an overview of the current state of plant genomics using a number of different approaches at a time when we celebrate the completion of the Arabidopsis genome sequence and begin the transition from structural to functional studies of this and other plant genomes. Topics covered include comparative genomics, computational approaches to gene identification and annotation and data management, high throughput methodologies for functional analysis at the levels of transcript, protein and metabolite, and methods for genome modification by both homologous and site-specific recombination. The book will provide a good introduction to some of the many aspects of genomics both for established plant biologists who wish to understand this rapidly developing area and for scientists early in their careers. It is also very suitable for a one-semester course in Plant Genomics at the upper-level undergraduate/graduate student level, where the individual chapters provide a framework that can be readily expanded by use of some of the many articles in the bibliographies.