Lanterna Rally

Author: Denisse G. Lopez

Publisher:

Published: 2016

Total Pages: 86

ISBN-13:

Cotton is the basic resource for thousands of consumer and industrial products manufactured in the world. Cotton seed meal (CSM) is a byproduct of the oil industry, and a source of high protein content, fiber and phosphorus. Cottonseed contains about 22.5% protein; this represents about 9.4 million tons of protein human consumption. Cottonseed meal is used for feeding cattle because of its high oil and protein content. After oil extraction cotton seed meal can have up to 55% protein; however, the presence of free gossypol may be responsible for poisoning in animals such as respiratory pain, anorexia, weakness, and death. Gossypol is found in the cotton plant and it is concentrated on cottonseed. There is currently a variety of Glandless Cotton Seed that contains a low amount of gossypol and can be processed into different food products for human consumption. The ability of glandless cottonseed meal to be processed into different foods is largely determined by the proteins that show the complexity and different interactions with each other. The objective of this research was to characterize the protein based on molecular weight, solubility, thermal stability and amino acid content. The deferent protein fractions in glandless cotton seed meal (GCSM) were extracted with a different solvent. The molecular weight of the different fraction was determined with SDS-PAGE. The GCSM protein had an isoelectric point of 5.1. No proteins were extracted with alcohol or acid solution. Most of the protein were extracted with 0.1 N salt solution and 0.1 N KOH solution. The glutenin fraction showed bands between 44 and 66 kDa and a denaturation temperature (Td) between 52.1 -- 71.7°C. The albumins had a molecular weight of 20 -- 29 kDa and a td between 50 -- 73.4°C. The globulins showed bands between 14 -- 20 kDa and a Td value of 53.6 -- 74.8°C. With this research, we can say that glandless cottonseed meal protein is as important as the most known proteins. Since glandless cottonseed meal contains similar values in fiber, minerals, amino acid and protein compared to soybean meal protein, whey protein, peanut meal protein and linseed protein we can use it as substitute or complement in the human diet.