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Cellular and Molecular Methods in Neuroscience Research

Author: Adalberto Merighi

Publisher: Springer Science & Business Media

Published: 2007-12-14

Total Pages: 307

ISBN-13: 0387224602

There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.

From Molecules to Networks

Author: Ruth Heidelberger

Publisher: Academic Press

Published: 2009-01-27

Total Pages: 654

ISBN-13: 0080920837

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An understanding of the nervous system at virtually any level of analysis requires an understanding of its basic building block, the neuron. From Molecules to Networks provides the solid foundation of the morphologic, biochemical, and biophysical properties of nerve cells. All chapters have been thoroughly revised for this second edition to reflect the significant advances of the past 5 years. The new edition expands on the network aspects of cellular neurobiology by adding a new chapter, Information Processing in Neural Networks, and on the relation of cell biological processes to various neurological diseases. The new concluding chapter illustrates how the great strides in understanding the biochemical and biophysical properties of nerve cells have led to fundamental insights into important aspects of neurodegenerative disease. Written and edited by leading experts in the field, the second edition completely and comprehensively updates all chapters of this unique textbook Discusses emerging new understanding of non-classical molecules that affect neuronal signaling Full colour, professional graphics throughout Includes two new chapters: Information Processing in Neural Networks - describes the principles of operation of neural networks and the key circuit motifs that are common to many networks in the nervous system. Molecular and Cellular Mechanisms of Neurodegenerative Disease - introduces the progress made in the last 20 years in elucidating the cellular and molecular mechanisms underlying brain disorders, including Amyotrophic Lateral Sclerosis (ALS), Parkinson disease, and Alzheimer’s disease

Cellular Imaging Techniques for Neuroscience and Beyond

Author: Floris G. Wouterlood

Publisher: Academic Press

Published: 2012-08-08

Total Pages: 298

ISBN-13: 0123858720

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In the biomedical sciences, the confocal laser scanning microscope (CLSM) has become the instrument of choice for producing high-resolution images and 3D reconstruction, breaking the barriers of conventional optical microscopy. Wouterlood (anatomy, VU U. Medical Center, Amsterdam, the Netherlands) introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin. The text includes color and b&w images, and referral to an online CLSM simulator. Academic Press is an imprint of Elsevier. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com).

Guide to Research Techniques in Neuroscience

Author: Matt Carter

Publisher: Academic Press

Published: 2022-03-26

Total Pages: 416

ISBN-13: 0323915612

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Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step

From Molecules to Networks

Author: John H. Byrne

Publisher: Academic Press

Published: 2014-05-23

Total Pages: 692

ISBN-13: 0123982677

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An understanding of the nervous system at virtually any level of analysis requires an understanding of its basic building block, the neuron. The third edition of From Molecules to Networks provides the solid foundation of the morphological, biochemical, and biophysical properties of nerve cells. In keeping with previous editions, the unique content focus on cellular and molecular neurobiology and related computational neuroscience is maintained and enhanced. All chapters have been thoroughly revised for this third edition to reflect the significant advances of the past five years. The new edition expands on the network aspects of cellular neurobiology by adding new coverage of specific research methods (e.g., patch-clamp electrophysiology, including applications for ion channel function and transmitter release; ligand binding; structural methods such as x-ray crystallography). Written and edited by leading experts in the field, the third edition completely and comprehensively updates all chapters of this unique textbook and insures that all references to primary research represent the latest results. The first treatment of cellular and molecular neuroscience that includes an introduction to mathematical modeling and simulation approaches 80% updated and new content New Chapter on "Biophysics of Voltage-Gated Ion Channels" New Chapter on "Synaptic Plasticity" Includes a chapter on the Neurobiology of Disease Highly referenced, comprehensive and quantitative Full color, professional graphics throughout All graphics are available in electronic version for teaching purposes

Short Protocols in Neuroscience

Author: Charles R. Gerfen

Publisher: Current Protocols

Published: 2006-08-04

Total Pages: 0

ISBN-13: 9780471783992

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Short Protocols in Neuroscience: Cellular and Molecular Methods is an easy-to-use compendium of cellular and molecular methods from Current Protocols in Neuroscience, part of the acclaimed series that has set the standard for excellence for laboratory manuals. The title provides a portable and streamlined at-the-bench resource, covering methods for analysis of neural cell structure and function, isolation and culture of neural cells, and characterization of neural cell gene expression.

Molecular Neuroscience

Author: Rusty Lansford

Publisher:

Published: 2014

Total Pages: 0

ISBN-13: 9781621820147

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A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.

Molecular and Cellular Approaches to Neural Development

Author: W. Maxwell Cowan

Publisher: Oxford University Press, USA

Published: 1997

Total Pages: 575

ISBN-13: 0195111664

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This text provides a broad but authoritative view of the cellular and molecular aspects of developmental neurobiology written by leaders in the field.

Cellular and Molecular Neurophysiology

Author: Constance Hammond

Publisher: Elsevier

Published: 2024-06-03

Total Pages: 602

ISBN-13: 0323986137

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Cellular and Molecular Neurophysiology, Fifth Edition is the only up-to-date textbook on the market that focuses on the molecular and cellular physiology of neurons and synapses. Hypothesis-driven rather than a dry presentation of the facts, the book promotes a real understanding of the function of nerve cells that is useful for practicing neurophysiologists and students in graduate-level courses on the topic alike. This new edition explains the molecular properties and functions of excitable cells in detail and teaches students how to construct and conduct intelligent research experiments. The content is firmly based on numerous experiments performed by top experts in the field. The new edition contains new chapters on recording neuronal activity, iconotrophic and metabotropic receptors for sensory transduction, and a section containing exercises for further learning. This book will be a useful resource for neurophysiologists, neurobiologists, neurologists, and students taking graduate-level courses on neurophysiology. Authoritative foundational coverage of basic cellular and molecular neurophysiology Includes new chapters on recording neuronal activity, iconotrophic and metabotropic receptors for sensory transduction Provides fifteen appendices that describe how neurobiological techniques are interspersed in the text Presents enhanced coverage of new methodologies and experimental techniques

Neuroscience Methods

Author: Rosemary Martin

Publisher: CRC Press

Published: 2019-08-16

Total Pages: 280

ISBN-13: 1000725359

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Unique in its coverage of such an extensive range of methods, Neuroscience Methods: A Guide for Advanced Students provides easy-to-understand descriptions of the many different techniques that are currently being used to study the brain at the molecular and cellular levels. This valuable reference text will help rescue undergraduate and postgraduate students from continuing bewilderment at the methods sections of current neuroscience publications. Topics covered include in vivo and in vitro preparations, electrophysiological, histochemical, hybridization and genetic techniques, measurement of cellular ion concentrations, methods of drug application, production of antibodies, expression systems, and neural grafting.