Biotechnology of Plant Secondary Metabolism: Methods and Protocols
Author: Arthur Germano Fett-Neto
Publisher: Methods in Molecular Biology
Published: 2018-04-15
Total Pages: 194
ISBN-13: 9781493980406
DOWNLOAD EBOOK →Author: Arthur Germano Fett-Neto
Publisher: Methods in Molecular Biology
Published: 2018-04-15
Total Pages: 194
ISBN-13: 9781493980406
DOWNLOAD EBOOK →Author: Arthur Germano Fett-Neto
Publisher: Humana
Published: 2022-05-05
Total Pages: 262
ISBN-13: 9781071621844
DOWNLOAD EBOOK →This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols. Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field.
Author: Ganesh Sriram
Publisher:
Published: 2014
Total Pages: 318
ISBN-13: 9781627036610
DOWNLOAD EBOOK →In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.
Author: Arthur Germano Fett-Neto
Publisher: Methods in Molecular Biology
Published: 2010-05-06
Total Pages: 360
ISBN-13:
DOWNLOAD EBOOK →This book presents detailed practical information on important methods used in the engineering of plant secondary metabolism pathways and the acquisition of essential knowledge in performing this activity, including important advances and emerging strategies.
Author: Mohd. Shahnawaz
Publisher: CRC Press
Published: 2021-11-05
Total Pages: 186
ISBN-13: 1000174948
DOWNLOAD EBOOK →Thousands of secondary metabolites are produced by plants to withstand unfavourable environmental conditions and are important molecules for nutraceutical, agro, cosmetic and pharmaceutical industries, etc. Harvesting of plants for the extraction of these important metabolites can threaten the plant germplasm, and various medicinally important plants are at the verge of extinction. Based on need, various methods and strategies were developed and followed by researchers from time to time to save the plant germplasm and produce important secondary metabolites efficiently to meet their growing demands. Biotechnological Approaches to Enhance Plant Secondary Metabolites: Recent Trends and Future Prospects provides a comprehensive introduction and review of state-of-the-art biotechnological tools in this field of research at global level. The methodologies are highlighted by real data examples in both in vitro and in vivo level studies. The book: • Highlights and provides overviews of the synthesis, classification, biological function and medicinal applications of the recent advancements for the enhanced production of novel secondary metabolites in plants • Provides an overview of the role of induced mutation, salinity stress and brassinosteroids impact to increase the secondary metabolic contents in plants and suggests an increase in enzymatic activity in plants could be due to various point mutations, which in turn could play a role at transcriptome levels • Discusses the significant role of endophytes to enhance the contents of plant secondary metabolites • Alternatively, suggests the urgent need to set up the standard operating procedures using hydroponics system of cultivation for significant enhancement of secondary metabolite contents • Enlists various in vitro techniques to enhance plant secondary metabolites contents using plant tissue culture approaches • Provides a systematic overview of state-of-the-art biotechnological tools CRISPER Cas9 and RNAi to enhance the plant secondary metabolite contents • Recommends CRISPER Cas9 technology over RNAi, ZFNs and TALENs because of its relatively simple and high precision method with an easily programmable tool This serves as a reference book for the researchers working in the field of plant secondary metabolites and pharmaceutical industries at global level.
Author: Carla António
Publisher: Humana
Published: 2018-05-15
Total Pages: 0
ISBN-13: 9781493978182
DOWNLOAD EBOOK →This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.
Author: Michael Wink
Publisher: John Wiley & Sons
Published: 2010-01-26
Total Pages: 424
ISBN-13: 1444318888
DOWNLOAD EBOOK →This important volume commences with an overview of the modes of action of defensive secondary metabolites, followed by detailed surveys of chemical defense in marine ecosystems, the biochemistry of induced defense, plant-microbe interactions and medical applications. A chapter is also included covering biotechnological aspects of producing valuable secondary metabolites in plant cell and organ cultures. This is a comprehensive and fully updated new edition, edited by Professor Michael Wink and including contributions from many internationally acknowledged experts in the field.
Author: Hiroshi Ashihara
Publisher: John Wiley & Sons
Published: 2010-03-11
Total Pages: 424
ISBN-13: 1119993229
DOWNLOAD EBOOK →Various plant metabolites are useful for human life, and the induction and reduction of these metabolites using modern biotechnical technique is of enormous potential important especially in the fields of agriculture and health. Plant Metabolism and Biotechnology describes the biosynthetic pathways of plant metabolites, their function in plants, and some applications for biotechnology. Topics covered include: biosynthesis and metabolism of starch and sugars lipid biosynthesis symbiotic nitrogen fixation sulfur metabolism nucleotide metabolism purine alkaloid metabolism nicotine biosynthesis terpenoid biosynthesis benzylisoquinoline alkaloid biosynthesis monoterpenoid indole alkaloid biosynthesis flavonoid biosynthesis pigment biosynthesis: anthocyanins, betacyanins and carotenoids metabolomics in biotechnology Plant Metabolism and Biotechnology is an essential guide to this important field for researchers and students of biochemistry, plant biology, metabolic engineering, biotechnology, food science, agriculture, and medicine.
Author: Ganesh Sriram
Publisher: Humana
Published: 2016-09-17
Total Pages: 0
ISBN-13: 9781493958818
DOWNLOAD EBOOK →In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.