Atlas of Hematologic Neoplasms

Atlas of Hematologic Neoplasms PDF

Author: Tsieh Sun

Publisher: Springer Science & Business Media

Published: 2009-06-12

Total Pages: 533

ISBN-13: 0387898484

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Due to its rapid development in recent years, hematopathology has become a very complicated discipline. The current development is mainly in two aspects: the new classification of lymphomas and leukemias and the new techniques. The Revised European-American Classification of Lymphoid Neoplasms (REAL classification) and the World Health Organization (WHO) classification of hematologic neoplasms require not only morphologic criteria but also immunophenotyping and molecular genetics for the diagnosis of hematologic tumors. Immunophenotyping is performed by either flow cytometry or immunohistochemistry. There are many new monoclonal antibodies and new equipments accumulated in recent years that make immunophenotyping more or more accurate and helpful. There are even more new techniques invented in recent years in the field of molecular genetics. In cytogenetics, the conventional karyotype is supplemented and partly replaced by the fluorescence in situ hybridization (FISH) technique. The current development of gene expression profiling is even more powerful in terms of subtyping the hematologic tumors, which may help guiding the treatment and predict the prognosis. In molecular biology, the tedious Southern blotting technique is largely replaced by polymerase chain reaction (PCR). The recent development in reverse-transcriptase PCR and quantitative PCR makes these techniques even more versatile. Because of these new developments, hematopathology has become too complicated to handle by a general pathologist. Many hospitals have to hire a newly trained hematopathologist to oversee peripheral blood, bone marrow and lymph node examinations. These young hematopathologists are geared to the new techniques, but most of them are inexperienced in morphology. No matter how well-trained a hematopathologist is, he or she still needs to see enough cases so that they can recognize the morphology and use the new techniques to substantiate the diagnosis. In other words, morphology is still the basis for the diagnosis of lymphomas and leukemias. Therefore, a good color atlas is the most helpful tool for these young hematopathologists and for the surgical pathologists who may encounter a few cases of hematologic tumors from time to time. In a busy daily practice, it is difficult to refer to a comprehensive hematologic textbook all the time. There are a few hematologic color atlases on the market to show the morphology of the normal blood cells and hematologic tumor cells. These books are helpful but not enough, because tumor cell morphology is variable from case to case and different kinds of tumor cells may look alike and need to be differentiated by other parameters. The best way to learn morphology is through the format of clinical case study. This format is also consistent with the daily practice of hematopathologists and with the pattern in all the specialty board examinations. Therefore, it is a good learning tool for the pathology residents, hematology fellows as well as medical students. This proposed book will present 83 clinical cases with clinical history, morphology of the original specimen and a list of differential diagnoses. This is followed by further testing with pictures to show the test results. At the end, a correct diagnosis is rendered with subsequent brief discussion on how the diagnosis is achieved. A few useful references will be cited and a table will be provided for differential diagnosis in some cases. The major emphasis is the provision of 500 color photos of peripheral blood smears, bone marrow aspirates, core biopsy, lymph node biopsy and biopsies of other solid organs that are involved with lymphomas and leukemias. Pictures of other diagnostic parameters, such as flow cytometric histograms, immunohistochemical stains, cytogenetic karyotypes, fluorescence in situ hybridization and polymerase chain reaction, will also be included. A comprehensive approach with consideration of clinical, morphologic, immunophenotypic and molecular genetic aspects is the best way to achieve a correct diagnosis. After reading this book, the reader will learn to make a diagnosis not only based on the morphology alone but also in conjunction with other parameters.

Flow Cytometry, Immunohistochemistry, and Molecular Genetics for Hematologic Neoplasms

Flow Cytometry, Immunohistochemistry, and Molecular Genetics for Hematologic Neoplasms PDF

Author: Tsieh Sun

Publisher: Lippincott Williams & Wilkins

Published: 2012-01-19

Total Pages: 449

ISBN-13: 1451180640

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Immunophenotyping is the most powerful tool in the routine diagnosis of hematologic neoplasms. Immunohistochemical technique is used in histology labs for this purpose, while flow cytometry is used in clinical labs. Although separately these 2 techniques are very useful in detecting lymphomas and leukemias, the combination of both creates a very powerful and definitive diagnostic tool. The addition of molecular genetics to the book makes it an all-encompassing reference text.

Flow Cytometric Analysis of Hematologic Neoplasms

Flow Cytometric Analysis of Hematologic Neoplasms PDF

Author: Tsieh Sun

Publisher: Lww

Published: 2002

Total Pages: 276

ISBN-13: 9780781729642

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This thoroughly updated Second Edition provides a comprehensive review of the basic principles, clinical applications, and interpretation of flow cytometry results. Focusing on hematologic neoplasms, it covers current uses and applications while considering cost containment trends in healthcare. More than 30 complete clinical cases with detailed clinical histories, flow cytometric findings, morphologic illustrations, and other pertinent information on molecular biological techniques, cytochemistry, and cytogenetics provide readers with complete clinical information to assist in diagnosis, as well as to hone skills. The text also features 6 new leukemias/lymphomas and 94 new color illustrations, including color photographs of peripheral blood smears, bone marrow smears, bone marrow core biopsies, and histologic sections of lymph nodes, spleen, liver and solid tumors.

Current Catalog

Current Catalog PDF

Author: National Library of Medicine (U.S.)

Publisher:

Published:

Total Pages: 1712

ISBN-13:

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First multi-year cumulation covers six years: 1965-70.

Hematology - E-Book

Hematology - E-Book PDF

Author: Bernadette F. Rodak

Publisher: Elsevier Health Sciences

Published: 2013-12-27

Total Pages: 885

ISBN-13: 0323292690

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Featuring hundreds of full-color photomicrographs, Hematology: Clinical Principles and Applications prepares you for a job in the clinical lab by exploring the essential aspects of hematology. It shows how to accurately identify cells, simplifies hemostasis and thrombosis concepts, and covers normal hematopoiesis through diseases of erythroid, myeloid, lymphoid, and megakaryocytic origins. This book also makes it easy to understand complementary testing areas such as flow cytometry, cytogenetics, and molecular diagnostics. Well-known authors Bernadette Rodak, George Fritsma, and Elaine Keohane cover everything from working in a hematology lab to the parts and functions of the cell to laboratory testing of blood cells and body fluid cells. Full-color illustrations make it easier to visualize complex concepts and show what you’ll encounter in the lab. Learning objectives begin each chapter, and review questions appear at the end. Instructions for lab procedures include sources of possible errors along with comments. Case studies provide opportunities to apply hematology concepts to real-life scenarios. Hematology instruments are described, compared, and contrasted. Coverage of hemostasis and thrombosis includes the development and function of platelets, the newest theories of normal coagulation, and clear discussions of platelet abnormalities and disorders of coagulation. A bulleted summary of important content appears at the end of every chapter. A glossary of key terms makes it easy to find and learn definitions. Hematology/hemostasis reference ranges are listed on the inside front and back covers for quick reference. Respected editors Bernadette Rodak, George Fritsma, and Elaine Keohane are well known in the hematology/clinical laboratory science world. Student resources on the companion Evolve website include the glossary, weblinks, and content updates. New content is added on basic cell biology and etiology of leukocyte neoplasias. Updated Molecular Diagnostics chapter keeps you current on techniques being used in the lab. Simplified hemostasis material ensures that you can understand this complex and important subject. Coverage of morphologic alteration of monocytes/macrophages is condensed into a table, as the disorders in this grouping are more of a biochemical nature with minimal hematologic evidence.

Handbook of Hematologic Pathology

Handbook of Hematologic Pathology PDF

Author: Harold Schumacher

Publisher: CRC Press

Published: 2019-09-17

Total Pages: 812

ISBN-13: 042952448X

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This handy reference demonstrates how to use blood, plasma, serum, instrumentation, bone marrow, lymph nodes, and splenic tissue to establish diagnosis, emphasizing practical information to aid in the operation of an efficient hematology laboratory and highlighting up-to-date investigative procedures. Supplies case studies to allow for self-tes