Antibody Arrays
Author: Kelly C. Whittaker
Publisher:
Published: 2021
Total Pages:
ISBN-13: 9781071610640
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Protein Arrays, Biochips and Proteomics
Author: Joanna S. Albala
Publisher: CRC Press
Published: 2003-08-20
Total Pages: 422
ISBN-13: 0824756347
DOWNLOAD EBOOK →From disease marker identification to accelerated drug development, Protein Arrays, Biochips, and Proteomics offers a detailed overview of current and emerging trends in the field of array-based proteomics. This reference focuses on innovations in protein microarrays and biochips, mass spectrometry, high-throughput protein expression, protein-protein interactions, structural proteomics, and the proteomic marketplace for comprehensive understanding of past, present, and future proteomic research. Offering an abundance of figures and charts, the book compiles a wide variety of technologies and applications ranging from functionalized chip surfaces to strategies for protein expression.
Protein Arrays
Author: Eric Fung
Publisher: Springer Science & Business Media
Published: 2008-02-03
Total Pages: 279
ISBN-13: 1592597599
DOWNLOAD EBOOK →Protein Arrays: Methods and Protocols is an introduction to protein array technology and its application to the multiplexed detection of proteins. Although protein array technology has some roots in gene array technology, it can only be described as a distant relative. Unlike DNA, with its established rules of base pairing, and therefore predictable biochemical behavior, proteins are rich with diversity. Proteins can be large or small, compact or extended, basic or acidic, hydrophobic or hydrophilic, and so on. Just as importantly, their behavior is determined by the environment in which they reside, and so the composition of the buffer in which experiments are performed has a dramatic impact on the outcome of the experiment. Thus, if the goal is to simultaneously measure the expression of a large number of proteins, these variables must be addressed. Not to be deterred, scientists have created a variety of solutions to successfully detect and ch- acterize multiple proteins simultaneously. It is the intent of this volume to introduce to the reader a set of technological solutions to the diversity problem as well as to provide the reader with some examples of practical applications of these technologies.
Microarray Technology in Practice
Author: Steve Russell
Publisher: Academic Press
Published: 2008-11-21
Total Pages: 460
ISBN-13: 0080919766
DOWNLOAD EBOOK →Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique. Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments Up-to-date description of normalization methods and current methods for sample amplification and labeling Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays
Microarrays
Author: Jang B. Rampal
Publisher: Springer Science & Business Media
Published: 2008-02-03
Total Pages: 927
ISBN-13: 1597453048
DOWNLOAD EBOOK →This second part in the two-volume work Microarrays details applications and data analysis. It includes insight into non-mammalian vertebrate systems, processes and protocols for high quality glass-based microarrays. Coverage includes applications in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray platforms in clinical practice, and screening of cDNA libraries on glass slide microarrays. Authors in this volume also discuss protocols for predicting DNA duplex stability on oligonucleotide arrays and integrated analysis of microarray results.
Reverse Phase Protein Arrays
Author: Tesshi Yamada
Publisher: Springer Nature
Published: 2019-12-09
Total Pages: 264
ISBN-13: 9813297557
DOWNLOAD EBOOK →This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.
Immunogenomics and Human Disease
Author: Andras Falus
Publisher: John Wiley & Sons
Published: 2006-03-30
Total Pages: 548
ISBN-13: 047003324X
DOWNLOAD EBOOK →This book provides an overview of key conceptual and molecular technologies being deployed in immunogenomics, followed by detailed evaluations of the impact of genomics and systems biology on important areas such as cancer immunology, autoimmunity, allergy and the response to infection.
Microarray Methods and Protocols
Author: Robert S. Matson
Publisher: CRC Press
Published: 2009-01-20
Total Pages: 216
ISBN-13: 1420046667
DOWNLOAD EBOOK →A Step-by-Step Guide to Present and Future Uses of Microarray TechnologyMicroarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts
New Antibody Microarray Tube for Cellular Localization and Signaling Pathways
Author: Lin Wang
Publisher: Springer Science & Business Media
Published: 2009-05-27
Total Pages: 139
ISBN-13: 3540743413
DOWNLOAD EBOOK →This book introduces a new biochip that incorporates cellular localization and bioinformatics technologies. With the post-genome era, proteomics study for direct analysis of a group of proteins by biochip becomes more and more important. With this book and its 50 tables and figures, the reader can not only learn about new technologies but also about cellular localization, signaling pathways, and protein relationships.
Proteomics in Biomedicine and Pharmacology
Author:
Publisher: Academic Press
Published: 2014-07-03
Total Pages: 377
ISBN-13: 0128005955
DOWNLOAD EBOOK →Volume 95 of Advances in Protein Chemistry and Structural Biology focuses on advances in proteomic techniques and their application in biomedicine and pharmacology. This volume describes in detail the applications of these techniques in studying a number of diseases and specific protein modifications and profiles, including phosphoproteomics, glycoproteomics, and more. Published continuously since 1944, the Advances in Protein Chemistry and Structural Biology series is the essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins. Each thematically organized volume is guest edited by leading experts in a broad range of protein-related topics. Describes advances in application of powerful techniques in translational research (biomedicine and pharmacology) Chapters are written by authorities in their field Targeted to a wide audience of researchers, specialists, and students The information provided in the volume is well supported by a number of high quality illustrations, figures, and tables
