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Animal Cell Biotechnology

Author: Ralf Pörtner

Publisher: Humana

Published: 2016-08-23

Total Pages: 0

ISBN-13: 9781493963188

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Animal Cell Biotechnology

Author: Ralf Pörtner

Publisher: Springer Science & Business Media

Published: 2007-04-05

Total Pages: 1035

ISBN-13: 1588296601

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The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The chapters are written by world-renowned experts and the volume’s five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.

Biotechnology-5: Animal Cells, Immunology & Plant Biotechnology

Author: M. K. Sateesh

Publisher: New Age International

Published: 2003

Total Pages: 214

ISBN-13: 9788122414516

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This Book Is Designed As Per The Syllabus Of Biotechnology Paper-5 Prescribed By Bangalore University And Other Indian Universities. The Book Is Divided Into Three Parts As Follows: * Animal Cell Biotechnology * Immunology * Plant BiotechnologyThe Presentation In Each Part Is Simple And Systematic. The Basic Concepts Have Been Clearly Explained And Their Functions Are Adequately Highlighted. A Few Recent Developments Have Also Been Included To Provide A Contemporary Understanding Of The Subject.

Animal Cell Biotechnology

Author: Nigel Jenkins

Publisher: Humana Press

Published: 1999-02-22

Total Pages: 302

ISBN-13: 9780896035478

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Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.

Animal Biotechnology

Author: Ashish S. Verma

Publisher: Academic Press

Published: 2013-11-04

Total Pages: 685

ISBN-13: 0123914345

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Animal Biotechnology introduces applications of animal biotechnology and implications for human health and welfare. It begins with an introduction to animal cell cultures and genome sequencing analysis and provides readers with a review of available cell and molecular tools. Topics here include the use of transgenic animal models, tissue engineering, nanobiotechnology, and proteomics. The book then delivers in-depth examples of applications in human health and prospects for the future, including cytogenetics and molecular genetics, xenografts, and treatment of HIV and cancers. All this is complemented by a discussion of the ethical and safety considerations in the field.Animal biotechnology is a broad field encompassing the polarities of fundamental and applied research, including molecular modeling, gene manipulation, development of diagnostics and vaccines, and manipulation of tissue. Given the tools that are currently available and the translational potential for these studies, animal biotechnology has become one of the most essential subjects for those studying life sciences. Highlights the latest biomedical applications of genetically modified and cloned animals with a focus on cancer and infectious diseases Provides firsthand accounts of the use of biotechnology tools, including molecular markers, stem cells, and tissue engineering

Animal Cell Biotechnology

Author: Nigel Jenkins

Publisher:

Published: 1999

Total Pages:

ISBN-13:

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Animal Cell Biotechnology

Author: Hansjörg Hauser

Publisher: Walter de Gruyter GmbH & Co KG

Published: 2014-11-10

Total Pages: 718

ISBN-13: 3110278960

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This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.

Animal Cell Biotechnology

Author: Hansjörg Hauser

Publisher: Walter de Gruyter GmbH & Co KG

Published: 2014-11-10

Total Pages: 715

ISBN-13: 3110381427

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Animal Biotechnology

Author: National Research Council

Publisher: National Academies Press

Published: 2002-12-29

Total Pages: 201

ISBN-13: 0309084393

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Genetic-based animal biotechnology has produced new food and pharmaceutical products and promises many more advances to benefit humankind. These exciting prospects are accompanied by considerable unease, however, about matters such as safety and ethics. This book identifies science-based and policy-related concerns about animal biotechnologyâ€"key issues that must be resolved before the new breakthroughs can reach their potential. The book includes a short history of the field and provides understandable definitions of terms like cloning. Looking at technologies on the near horizon, the authors discuss what we know and what we fear about their effectsâ€"the inadvertent release of dangerous microorganisms, the safety of products derived from biotechnology, the impact of genetically engineered animals on their environment. In addition to these concerns, the book explores animal welfare concerns, and our societal and institutional capacity to manage and regulate the technology and its products. This accessible volume will be important to everyone interested in the implications of the use of animal biotechnology.

Production of Biologicals from Animal Cells in Culture

Author: R. E. Spier

Publisher: Butterworth-Heinemann

Published: 2013-09-24

Total Pages: 853

ISBN-13: 1483165159

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Production of Biologicals from Animal Cells in Culture reviews the state of the art in animal cell biotechnology, with emphasis on the sequence of events that occur when generating a biological from animal cells in culture. Methods that enable adjustment of nutrient feed streams into perfusion bioreactors so as to increase productivity are described. A number of issues are also addressed, such as the usefulness of the fingerprint method for cell characterization. Comprised of 135 chapters, this book begins with an overview of the problems and benefits of animal cell culture, followed by a discussion on the isolation of immortal murine macrophage cell lines. The reader is systematically introduced to the use of DNA fingerprinting to characterize cell banks; immortalization of cells with oncogenes; lipid metabolism of animal cells in culture; and energetics of glutaminolysis. Subsequent chapters explore serum-free and protein-free media; the physiology of animal cells; gene expression in animal cell systems; and animal cell bioreactors. The monitoring and assay of animal cell parameters are also considered, along with downstream processing and regulatory issues. This monograph will be of interest to students, practitioners, and investigators in the fields of microbiology and biotechnology.