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Author: K.J. Puite
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 415
ISBN-13: 9400927886
DOWNLOAD EBOOK →Proceedings of the 7th International Protoplast Symposium, Wageningen, The Netherlands, December 6-11, 1987
Author: Potrykus
Publisher: Birkhäuser
Published: 1983-01-01
Total Pages: 396
ISBN-13: 9783764315139
DOWNLOAD EBOOK →Protoplast culture: preparatory studies; Protoplast culture: induction of sustained divisions; Protoplast culture: plants from protoplast - derived clones; Protoplast fusion and somatic hybridisation; Cytoplasmic hybridisation and organelle transfer; Genetic transformation; Somaclonal variation; Mutants and stable variants; Protoplasts for studies in virology; Plant microbe interactions; Physiological studies; Cytology and ultrastructure; Protoplast as tools in biotechnology; The use of protoplasts of study development; Gene expression in protoplast; Miscellaneous; Novel techniques; Protoplast technology for plant breeding; Microbial protoplasts; Protoplast isolation and fusion techniques; Protoplast fusion and genetic analysis; Morphological studies.
Author: John F. Peberdy
Publisher: CRC Press
Published: 2020-10-28
Total Pages: 369
ISBN-13: 1000110591
DOWNLOAD EBOOK →Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.
Author: Ingo Potrykus
Publisher: Birkhäuser
Published: 1983
Total Pages: 280
ISBN-13:
DOWNLOAD EBOOK →Recent developments in the culture and regeneration of plant protoplasts; Protoplast culture and plant regeneration of cereals and other recalcitrant crops; Protoplasts and the isolation of plant mutants; Protoplasts and variation from culture; Systems for plant protoplast transformation; Recent developments in plant protoplast fusion and selection technology; Somatic hybridization by plant protoplast fusion; The segregation of organelles and cytoplasmic traits in higher plant somatic fusion hybrids; The first mitotic cycle of mesophyll protoplasts; Protoplasts for studies of the plasma membrane and associated cell organelles; The use of protoplasts in plant virus research; Applications of protoplast technology to agriculture; Organelle transfer, sorting out, recombination; Plant protoplasts as tools for physiological studies; Genetic transformation; Protoplasts as tools in pathology, virology and plant-microbe interactions; Current questions of gene transfer via protoplast fusion in microorganisms (Opening adress); Advances in protoplast fusion and transformation in Streptomyces; Chromosome interactions and expression in fused Bacillus protoplasts; Yeast strain improvement by protoplast fusion and transformation; Protoplasts of filamentous fungi in genetics and metabolite production; Interspecific somatic hybridisation in Aspergillus; Synthesis and assembly of wall polymers on regenerating yeast protoplasts; The theory and practical applications of liposome-protoplast interactions; Transport of nutrients in yeast protoplast; Protoplasts and spheroplasts of gram-negative bacteria - with special emphasis on proteus mirabilis; Characteristic properties and biological significance of stable protoplast type L-forms; Advances in microbial protoplasts (Bacillus licheniformis lactamase and the protoplast surface); Fungal protoplasts as genetic tools; Non-complementing diploids: prokaryotic microorganism protoplast fusion and chromosome inactivation.
Author: Kan Wang
Publisher: Humana
Published: 2023-03-23
Total Pages: 0
ISBN-13: 9781071621660
DOWNLOAD EBOOK →This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.
