-PDF Download- Advanced Protocols For Animal Transgenesis EBOOK

Advanced Protocols for Animal Transgenesis

Author: Shirley Pease

Publisher: Springer Nature

Published: 2011-08-30

Total Pages: 671

ISBN-13: 3642207928

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Advanced Protocols for Animal Transgenesis

Author: Shirley Pease

Publisher: Springer

Published: 2016-08-23

Total Pages: 669

ISBN-13: 9783662519431

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Transgenesis Techniques

Author: Alan R. Clarke

Publisher: Springer Science & Business Media

Published: 2008-02-05

Total Pages: 346

ISBN-13: 1592591787

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The past decade has witnessed a spectacular explosion in both the devel- ment and use of transgenic technologies. Not only have these been used to aid our fundamental understanding of biologic mechanisms, but they have also faci- tated the development of a range of disease models that are now truly beginning to impact upon our approach to human disease. Some of the most exciting model systems relate to neurodegenerative disease and cancer, where the availability of appropriate models is at last allowing radically new therapies to be developed and tested. This latter point is of particular significance given the current concerns of the wider public over both the use of animal models and the merits of using genetically modified organisms. Arguably, advances of the greatest significance have been made using mammalian systems—driven by the advent of embryonic stem-cell–based strategies and, more recently, by cloning through nuclear transfer. For this reason, this new edition of Transgenesis Techniques focuses much more heavily on manipulation of the mammalian genome, both in the general discussions and in the provision of specific protocols.

Transgenic Animal Technology

Author: Carl A. Pinkert

Publisher: Gulf Professional Publishing

Published: 2002-11-18

Total Pages: 635

ISBN-13: 0125571666

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A transgenic animal is one that is genetically modified to carry genes from another species. Transgenic species can be raised to carry potentially useful genes from a variety of species. While the topics of genetic engineering and cloning are controversial, the reality is that these technologies offer tremendous benefits to society - from offering a framework for developing and screening medical therapies, to enhancing the safety and nutrition of the food we eat. One potential application of research into transgenic animal technology is the creation of domestic animals genetically designed to express a certain human disease and therefore serve as models for the study and treatment of human illnesses. Although many mouse models of human diseases are available today, such models in large domestic animals physiologically more similar to humans are sparse and critically needed. Further research in this field will undoubtedly uncover many more direct and indirect benefits of this technology. Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols New section on analysis

Transgenesis Techniques

Author: Alan R. Clarke

Publisher:

Published: 2002

Total Pages:

ISBN-13:

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This revised and updated edition of a much-acclaimed classic presents a collection of readily reproducible techniques for the generation and analysis of transgenic animals, with emphasis on the manipulation of the mammalian genome. It concentrates on the creation and maintenance of genetically modified murine strains, providing access to the germline by both conventional pronuclear injection, and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms.

Animal Transgenesis and Cloning

Author: Keywon Frazier

Publisher: Scientific e-Resources

Published: 2018-09-27

Total Pages: 276

ISBN-13: 1839474432

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Transgenic methodologies continue to evolve and have dramatically influenced a cross section of disciplines. They are recognized as instrumental in expanding our understanding of gene expression, regulation and function. This book covers the aspects of gene transfer in animals-from molecular methods to whole animal considerations across a host of species. The book starts with an introduction of what are transgenic animals. Chapter 1 methods and applications related to transgenic application. Chapter 2 describes the Use of Transgenic Animals in Biotechnology as Prospects and Problems. Chapter 3 study about Transgenic Animals in Agriculture. Chapter 4 depicts about the Gene Replacement and Transgenic Animals. This chapter give insight on Specific Sites in Cloned Genes Can Be Altered in Vitro and DNA that can be transferred into Eukaryotic Cells in Various Ways. Chapter 5 discuss about basics of cloning. Chapter 6 tells about the Reproductive Cloning. Chapter 7 tells about the Cloning of Domestic Animals. Chapter 8 depicts about the Surface Epigenetic Reprogramming. Chapters 9 devoted to Animal Health Risks. This chapter focus on the critical biological systems approach to the analysis of clone animal. Chapter 10 describes the development of the Risk Assessment Methodology required for cloning.

Transgenic Mouse Methods and Protocols

Author: Marten H. Hofker

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 382

ISBN-13: 1592593402

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Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.

Animal Biotechnology

Author: National Research Council

Publisher: National Academies Press

Published: 2002-12-29

Total Pages: 201

ISBN-13: 0309084393

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Genetic-based animal biotechnology has produced new food and pharmaceutical products and promises many more advances to benefit humankind. These exciting prospects are accompanied by considerable unease, however, about matters such as safety and ethics. This book identifies science-based and policy-related concerns about animal biotechnologyâ€"key issues that must be resolved before the new breakthroughs can reach their potential. The book includes a short history of the field and provides understandable definitions of terms like cloning. Looking at technologies on the near horizon, the authors discuss what we know and what we fear about their effectsâ€"the inadvertent release of dangerous microorganisms, the safety of products derived from biotechnology, the impact of genetically engineered animals on their environment. In addition to these concerns, the book explores animal welfare concerns, and our societal and institutional capacity to manage and regulate the technology and its products. This accessible volume will be important to everyone interested in the implications of the use of animal biotechnology.

Transgenic Animals in Agriculture

Author: James Dickson Murray

Publisher: Cabi

Published: 1999

Total Pages: 312

ISBN-13:

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In the past decade, a number of advances have been made in genetic engineering as applied to farmed animals. This book has been developed from invited presentations at a conference held in California in August 1997, to address this issue. It is written by representatives from the leading laboratories involved in attempts to improve agriculturally important mammals, poultry and fish. Current knowledge, methodology, technical improvements and successes in the applications of transgenic technology to a range of animals which are important in agriculture are brought together for the first time under one cover. This book is essential reading for research workers in animal genetics, breeding and biotechnology.

Animal Transgenesis and Cloning

Author: Louis-Marie Houdebine

Publisher: Wiley

Published: 2003-04-02

Total Pages: 234

ISBN-13: 9780470848289

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Animal Transgenesis and Cloning is a concise, balanced introduction to this dynamic subject, covering key issues and current techniques currently used in animal transgenesis and cloning. Whilst providing the reader with the essentials of the subject, from the molecular basis of gene structure and function to therapeutic gene cloning in humans, social and ethical implications of this important area of research are also considered. Written in a clear, accessible style, the book starts with an introduction to key molecular biology techniques and in particular, considers techniques used specifically for cloning animals and generating transgenic animals. Later chapters examine the diverse theoretical, technical and ethical issues raised by cloning and transgenesis in both animals and humans. Animal Transgenesis and Cloning: * Is an accessible, concise introduction to this dynamic field * Discusses biological, technical and ethical issues related to cloning and transgenesis * Provides an up-to-date account of the latest research methods and applications. Reviews 'This is a proposal for a book translation in a field that is clearly of importance and very topical. The idea of the book, from gene to transgenic animal, to its uses and abuses is sound and logical ...... It is probable that I would suggest (using) a book similar to the one proposed' Dr Janet Smith, University of Birmingham. 'Chapter one was a tour de force! Very concise and admirably clear ...... I was particularly impressed with the author's 2-page summary of eukaryotic gene regulation, which is no mean achievment!' Dr David de Pomerai, University of Nottingham. '...... I felt that this is a well written, topical book. It covers a lot of ground ...... in my opinion the author manages to provide a satisfying amount of detail and a well focussed treatment of each topic. I like the logic of the book, the fact that it pulls together all of the issues into one readable and manageable text' Dr Brendan Curran, Queen Mary, University of London.